A Distal CCAAT/NUCLEAR FACTOR Y Complex Promotes Chromatin Looping at the FLOWERING LOCUS T Promoter and Regulates the Timing of Flowering in Arabidopsis
| Autor: | Shuanghe Cao, Roderick W. Kumimoto, Roberto Mantovani, Ben F. Holt, Alessandra Maria Calogero, Nerina Gnesutta |
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| Rok vydání: | 2014 |
| Předmět: |
photoperiodism
Genetics Proximal promoter biology Arabidopsis Proteins fungi Arabidopsis CAAT box food and beverages Locus (genetics) Flowers Cell Biology Plant Science biology.organism_classification Chromatin CCAAT-Binding Factor Promoter activity Distal Enhancer Elements Promoter Regions Genetic Research Articles |
| Zdroj: | The Plant Cell. 26:1009-1017 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.113.120352 |
| Popis: | For many plant species, reproductive success relies on the proper timing of flowering, and photoperiod provides a key environmental input. Photoperiod-dependent flowering depends on timely expression of FLOWERING LOCUS T (FT); however, the coordination of various cis-regulatory elements in the FT promoter is not well understood. Here, we provide evidence that long-distance chromatin loops bring distal enhancer elements into close association with the proximal promoter elements bound by CONSTANS (CO). Additionally, we show that NUCLEAR FACTOR Y (NF-Y) binds a CCAAT box in the distal enhancer element and that CCAAT disruption dramatically reduces FT promoter activity. Thus, we propose the recruitment model of photoperiod-dependent flowering where NF-Y complexes, bound at the FT distal enhancer element, help recruit CO to proximal cis-regulatory elements and initiate the transition to reproductive growth. |
| Databáze: | OpenAIRE |
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