Identification of osteopontin in isolated rabbit osteoclasts
| Autor: | Takuya Sato, Tetsu Matsuo, Mitsue Ohta, Kenichi Tezuka, Yoshiyuki Hakeda, Kayo Tanaka, Peter J. Nijweide, Noriyoshi Kurihara, Hiroshi Kamioka, Masayoshi Kumegawa |
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| Rok vydání: | 1992 |
| Předmět: |
musculoskeletal diseases
Sialoglycoproteins Molecular Sequence Data Biophysics Osteoclasts Biology Biochemistry Bone and Bones Bone resorption Bone remodeling Tissue culture Osteoclast Sequence Homology Nucleic Acid medicine Animals Humans Amino Acid Sequence RNA Messenger Osteopontin Molecular Biology Cells Cultured Gene Library cDNA library DNA Cell Biology Molecular biology In vitro Resorption medicine.anatomical_structure biology.protein RNA Rabbits DNA Probes Poly A |
| Zdroj: | Biochemical and Biophysical Research Communications. 186:911-917 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(92)90832-6 |
| Popis: | Bone remodeling is a complex process coupling bone formation and resorption. Osteoblasts, the bone-forming cells, are known to produce various bone matrix proteins and cytokines; however, little is known about protein factors produced by osteoclasts or bone-resorbing cells. A method utilizing the high affinity of osteoclasts for tissue culture dishes was developed to isolate a large number of pure osteoclasts from rabbit long bones. A cDNA library was then constructed from these isolated osteoclasts, and differential cDNA screening was performed between osteoclasts and spleen cells. Two clones representing osteoclast-specific clones, named OC-1 and OC-2, were isolated. By Northern blot analysis, OC-1 was expressed in osteoclasts and in kidneys, whereas OC-2 was specific for osteoclasts. OC-1 was found to encode osteopontin from its nucleotide sequence, and therefore, osteopontin may have other functions for osteoclastic bone resorption besides osteoclast attachment to bone. |
| Databáze: | OpenAIRE |
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