Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction

Autor: Beatriz Araujo Oliveira, Lea Campos de Oliveira, Franciane Mendes de Oliveira, Geovana Maria Pereira, Regina Maia de Souza, Erika Regina Manuli, Fabricio Klerynton Marchini, Evelyn Patrícia Sanchez Espinoza, Marcelo Park, Leandro Taniguchi, Pedro Vitale Mendes, Lucas Augusto Moyses Franco, Ana Catharina Nastri, Maura Salaroli de Oliveira, José Mauro Vieira Junior, Esper Georges Kallas, Anna Sara Levin, Ester Cerdeira Sabino, Silvia Figueiredo Costa
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Revista do Instituto de Medicina Tropical de São Paulo, Volume: 64, Article number: e63, Published: 30 SEP 2022
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e63
Revista do Instituto de Medicina Tropical de São Paulo; v. 64 (2022); e63
Revista do Instituto de Medicina Tropical de São Paulo
Instituto de Medicina Tropical (IMT)
instacron:IMT
ISSN: 1678-9946
0036-4665
Popis: COVID-19 disease is spread worldwide and diagnostic techniques have been studied in order to contain the pandemic. Immunochromatographic (IC) assays are feasible and a low-cost alternative especially in low and middle-income countries, which lack structure to perform certain diagnostic techniques. Here we evaluate the sensitivity and specificity of eleven different IC tests in 145 serum samples from confirmed cases of COVID-19 using RT-PCR and 100 negative serum samples from blood donors collected in February 2019. We also evaluated the cross-reactivity with dengue using 20 serum samples from patients with confirmed diagnosis for dengue collected in early 2019 through four different tests. We found high sensitivity (92%), specificity (100%) and an almost perfect agreement (Kappa 0.92) of IC assay, especially when we evaluated IgG and IgM combined after 10 days from the onset of symptoms with RT-PCR. However, we detected cross-reactivity between dengue and COVID-19 mainly with IgM antibodies (5 to 20% of cross-reaction) and demonstrated the need for better studies about diagnostic techniques for these diseases.
Databáze: OpenAIRE
Externí odkaz: