Effect of cholesterol in membranes. Pulsed nuclear magnetic resonance measurements of lipid lateral diffusion
| Autor: | Lennart B.-Å. Johansson, Göran Lindblom, Gösta Arvidson |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Magnetic Resonance Spectroscopy
food.ingredient Chemistry Cholesterol Diffusion Bilayer Lipid Bilayers Phospholipid Biochemistry Lecithin Kinetics chemistry.chemical_compound food Membrane Nuclear magnetic resonance Phase (matter) Phosphatidylcholines lipids (amino acids peptides and proteins) Lipid bilayer |
| Zdroj: | Biochemistry. 20:2204-2207 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00511a020 |
| Popis: | Lateral diffusion coefficients of lipids in a bilayer can be measured directly in a macroscopically aligned sample by use of a pulsed NMR method with pulsed magnetic field gradients [Lindblom, G., & Wennerstrom, H. (1977) Biophys. Chem. 6, 167]. This technique has been utilized to investigate the influence of cholesterol on the lipid diffusion of egg yolk lecithin, palmitoyloleoyllecithin, and dioleoyllecithin. It is found that cholesterol has a very small effect on the phospholipid diffusion. On the other hand, cholesterol has a great influence on the molecular ordering in the bilayer and on the lipid phase structure. It is therefore suggested that cholesterol exerts its dominant effect on the lipid membrane stability. |
| Databáze: | OpenAIRE |
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