Androgen receptor in genital tubercle of rabbit fetuses and newborns. Ontogeny and properties
| Autor: | Michel Berger, Marc De Turckheim, Georges Veyssiere, Christiane Jean-Faucher, C. Jean |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
medicine.medical_specialty Receptor complex Hot Temperature Sex Differentiation medicine.drug_class Genitalia Male In Vitro Techniques Biology Tritium urologic and male genital diseases Biochemistry Fetus Sex Factors Endocrinology Pregnancy Internal medicine Centrifugation Density Gradient medicine Animals Receptor Genital tubercle Sexual differentiation Androgen binding Binding protein Genitalia Female Androgen Androgen receptor Animals Newborn Receptors Androgen Pronase Female Rabbits |
| Zdroj: | Journal of Steroid Biochemistry. 23:399-404 |
| ISSN: | 0022-4731 |
| Popis: | In newborn rabbits of both sexes, an androgen receptor was characterized in the genital tubercle. Homogenates exhibited high affinity (Kd was about 0.4 nM) and saturable binding of [3H]methyltrienolone. The half-life of the [3H]5 alpha-dihydrotestosterone-androgen receptor complex was 72 h at 4 degrees C. The receptor was inactivated by heat and pronase and the binding was specific for potent androgens. Sucrose gradient analysis revealed a 8-9 S [3H]methyltrienolone binding protein in cytosols from both sexes. Androgen binding, in the homogenate, was detected as soon as day 18 of gestation in both sexes and the number of binding sites increased until birth. During sexual organogenesis and at birth there were no major differences between males and females in the amount or affinity of androgen binding. Specific androgen binding was also detected in sexual ducts of male and female newborns. |
| Databáze: | OpenAIRE |
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