Polymer monoliths with silver nanoparticles-cholesterol conjugate as stationary phases for capillary liquid chromatography
| Autor: | Bogusław Buszewski, Michał Szumski, Damian Grzywiński |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Thermogravimetric analysis
Glycidyl methacrylate Silver Polymers 02 engineering and technology 01 natural sciences Biochemistry Silver nanoparticle Polymerization Analytical Chemistry chemistry.chemical_compound Phase (matter) Triethylene glycol chemistry.chemical_classification Chromatography 010401 analytical chemistry Organic Chemistry Azobisisobutyronitrile General Medicine Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences Cholesterol chemistry Nanoparticles 0210 nano-technology Porosity Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 1526:93-103 |
| ISSN: | 0021-9673 |
| Popis: | Monoliths in fused-silica capillaries were prepared by an in-situ polymerization using glycidyl methacrylate (GMA) as a functional monomer and in the presence of different crosslinkers such as ethylene dimethacrylate (EDMA), trimethylolpropane trimethacrylate (TRIM), 1,6-hexanediol dimethacrylate (HEDMA), pentaerythritol tetraacrylate (PETeA), triethylene glycol dimethacrylate (TriEDMA), while azobisisobutyronitrile (AIBN) was an initiator. The monoliths' surfaces were modified chemically with cystamine dihydrochloride, followed by immobilization of Ag-NPs the surface of which was functionalized with cholesterol cysteamine (Chol-SH). Such a new material enabled the separation of proteins in a reversed phase mode. The influence of the kind of a cross-linker and composition of the porogenic solutions on the porosity and separation properties of the obtained polymers was investigated and discussed. It was found that the amount of Ag-NPs embedded on the monoliths' surfaces depended on the porous properties of the monoliths, which was directly connected with the kind of the cross-linkers used. For the determination of the amount of the attached Ag-NPs the thermogravimetric analysis was used and additionally the ISEC method as a comparative method was also proposed. It was found that the maximum amount (about 20% by weight) of the 10nm Ag-NPs was attached to the poly(GMA-EDMA) and poly(GMA-TRIM) monoliths. |
| Databáze: | OpenAIRE |
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