Screening and detection of human enterovirus 71 infection by a real-time RT-PCR assay in Marseille, France, 2009–2011
| Autor: | Rémi N. Charrel, G. Gonfrier, Laetitia Ninove, Audrey Dubot-Pérès, X. de Lamballerie, C.Y.Q. Tan, Christine Zandotti |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Microbiology (medical) foot and mouth disease Genome Viral Biology medicine.disease_cause genogroup C2 viruses Cell Line Microbiology Feces Genetic similarity genogroup C2 Enterovirus Infections medicine Enterovirus 71 Animals Humans Mass Screening viruses Prospective Studies Child enterovirus 71 Phylogeny Enterovirus picornaviridae Foot-and-mouth disease Reverse Transcriptase Polymerase Chain Reaction Genetic Variation Infant General Medicine medicine.disease biology.organism_classification Virology Enterovirus A Human Human enterovirus Infectious Diseases Real-time polymerase chain reaction Child Preschool RNA Viral Capsid Proteins Female France Seasons hand |
| Zdroj: | Clinical Microbiology and Infection. 18:E77-E80 |
| ISSN: | 1198-743X |
| Popis: | Enterovirus-positive samples diagnosed in Marseille (January 2009 to September 2011) were screened for EV71 by real-time RT-PCR. EV71 was detected in three children below the age of 2 years with no history of overseas travel; two of these cases were associated with severe clinical presentation. Viruses demonstrated genetic similarity to other European genogroup C2 strains. Strain MRS/09/3663 complete sequencing revealed 97.6% identity across the entire genome with a 2008 Singapore isolate, without signs of possible recombination events. To our knowledge, this is the first detection of EV71 infection in Marseille, France, that confirms the current circulation of EV71 in France. |
| Databáze: | OpenAIRE |
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