Four New Compounds Obtained from Cultured Cells of Artemisia annua
| Autor: | Minghua Qian, Rongmin Yu, Jiachen Zi, Peijie Xiao, Jianhua Zhu, Chang Chen, Chuxin Liang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Artemisia annua Cell Culture Techniques Pharmaceutical Science Oxidative phosphorylation 010402 general chemistry Sesquiterpene lactone Hydroxylation 01 natural sciences Article Gas Chromatography-Mass Spectrometry Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound Biosynthesis Artemisia annua cultured cells arteannuin I arteannuin J biosynthesis selective hydroxylation lcsh:Organic chemistry 010608 biotechnology Drug Discovery medicine Physical and Theoretical Chemistry Artemisinin Cells Cultured Chromatography High Pressure Liquid chemistry.chemical_classification biology Molecular Structure Plant Extracts Organic Chemistry biology.organism_classification Artemisinins 0104 chemical sciences Enzyme chemistry Biochemistry Chemistry (miscellaneous) Molecular Medicine medicine.drug |
| Zdroj: | Molecules, Vol 22, Iss 12, p 2264 (2017) Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 22; Issue 12; Pages: 2264 |
| ISSN: | 1420-3049 |
| Popis: | Four new compounds obtained from cultured cells of Artemisia annua were reported. Products were detected by HPLC-ELSD/GC-MS and isolated by chromatographic methods. The structures of four new compounds, namely 6-hydroxy arteannuin I (1), 1-hydroxy arteannuin I (2), 2-hydroxy arteannuin J (3), and 14-hydroxy arteannuin J (4), were elucidated using their physico-chemical properties by NMR and MS data analyses. The results from the spontaneous oxidative experiment indicated that the biosynthesis of the new compounds was enzyme-catalyzed. Interestingly, the enzymes in the cultured cells of A. annua showed the abilities of substrate-selective and region-selective hydroxylation of the sesquiterpene lactone. Furthermore, the artemisinin contents were increased by 50% and 80% compared to the control group after the addition of arteannuin I/J to the suspension-cultured cells of A. annua under light and dark culture conditions, respectively. |
| Databáze: | OpenAIRE |
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