Dabigatran assessment in patients with acute complications using routine coagulation assays
| Autor: | Artur J. Szkotak, Linda Stang, Susan Nahirniak, Kenneth Butcher |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Thrombin time Fibrinogen Gastroenterology Antithrombins Dabigatran Therapeutic index Internal medicine medicine Humans Blood Coagulation Aged Aged 80 and over medicine.diagnostic_test business.industry Hematology General Medicine Middle Aged medicine.disease Thrombosis Coagulation beta-Alanine Benzimidazoles Female Blood Coagulation Tests business Discovery and development of direct thrombin inhibitors medicine.drug Partial thromboplastin time |
| Zdroj: | Blood Coagulation & Fibrinolysis. 25:426-434 |
| ISSN: | 0957-5235 |
| DOI: | 10.1097/mbc.0000000000000056 |
| Popis: | Complications while on dabigatran therapy, particularly bleeding and thrombosis, are occurring, and require laboratory assessment. The utility of routine coagulation assays has been previously evaluated in stable patients, but not those with acute complications. The purpose of this study was to determine how to employ routine coagulation assays to assess dabigatran in patients with acute complications. Seventeen patients on dabigatran presenting with various complications were evaluated. In addition, plasma samples with various fibrinogen levels were spiked in vitro with dabigatran ranging from low trough levels to the highest supratherapeutic concentrations reported (5000 ng/ml). INR, partial thromboplastin time (PTT), thrombin time (TT, Diagnostica Stago reagent), and fibrinogen were assayed and results compared to that of the Hemoclot Thrombin Inhibitors assay. Interference in the Clauss fibrinogen assay was assessed using a variety of commercial reagents. The majority of patients on dabigatran with acute complications demonstrated a significant negative bias in PTT results compared to normal plasma. TT remained highly sensitive to the presence of dabigatran (at least 10 ng/ml) under all circumstances investigated. There was wide variation in the sensitivity of commercial fibrinogen assays to dabigatran, with some even showing interference in the therapeutic range but this could be mitigated. The PTT is unreliable as a method for assessment of dabigatran in patients with acute complications. The TT assay is a simple and reliable alternative, particularly when combined with a fibrinogen level. |
| Databáze: | OpenAIRE |
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