Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405 : comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

Autor: Duncan J. Maskell, Thomas J. Inzana, Sonia Lacouture, Marc Stegger, Marcelo Gottschalk, Alexander W. Tucker, Liancheng Lei, Janine T. Bossé, Miriam Koene, Øystein Angen, David Harris, Paul R. Langford, Aloka B. Bandara, Yanwen Li, Matthew T. G. Holden, Liza Miriam Cohen, Olusegun Oshota, Peter Kuhnert, Brendan W. Wren, Andrew N. Rycroft
Přispěvatelé: University of St Andrews. School of Medicine, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. St Andrews Bioinformatics Unit, University of St Andrews. Infection and Global Health Division, Biotechnology and Biological Sciences Research Council (BBSRC), Biotechnology and Biological Sciences Research Council
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Swine
BRa
Genome
Actinobacillus Infections
Plasmid
serovar 8 reference genome
AA
amino acid [SNPs. Abbreviations]
Swine Diseases
Genetics
biology
630 Agriculture
Strain (biology)
Bacteriologie
Actinobacillus pleuropneumoniae
High-Throughput Nucleotide Sequencing
Bacteriology
Host Pathogen Interaction & Diagnostics
QR Microbiology
General Medicine
ATCC
BHI
amino acid
0605 Microbiology
Brain Heart Infusion
SNPs
Short Communications
QH426 Genetics
Pathogens and Epidemiology
Serogroup
Serovar 8 reference genome
American Type Culture Collection
Genetic variation
Animals
QH426
Gene
Illumina dye sequencing
Host Pathogen Interaction & Diagnostics
Whole genome sequencing
0604 Genetics
ATCC
American Type Culture Collection
Genetic Variation
DAS
Bacteriology
BHI
Brain Heart Infusion
SNPs. Abbreviations: AA
biology.organism_classification
Host Pathogen Interactie & Diagnostiek
SNPs. Abbreviations: AA
amino acid
QR
Bacteriologie
Host Pathogen Interactie & Diagnostiek
570 Life sciences
Genome
Bacterial
Zdroj: Microbial Genomics 7 (2021) 11
Bossé, Janine T; Li, Yanwen; Cohen, Liza Miriam; Stegger, Marc; Angen, Øystein; Lacouture, Sonia; Gottschalk, Marcelo; Lei, Liancheng; Koene, Miriam; Kuhnert, Peter; Bandara, Aloka B; Inzana, Thomas J; Holden, Matthew T G; Harris, David; Oshota, Olusegun; Maskell, Duncan J; Tucker, Alexander W; Wren, Brendan W; Rycroft, Andrew N and Langford, Paul R (2021). Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation. Microbial genomics, 7(11) Microbiology Society 10.1099/mgen.0.000687
Microbial Genomics
Microbial Genomics, 7(11)
ISSN: 3152-0103
2057-5858
DOI: 10.1099/mgen.0.000687
Popis: This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, BB/G018553/1, BB/S002103/1, and BB/S005897/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. Funding for LL provided by the ‘National Natural Science Foundation of China’ (No.31520103917). MTGH and DH were supported by the Wellcome Trust (grant number 098051). We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain. Publisher PDF
Databáze: OpenAIRE
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