AC Electroosmosis-Enhanced Nanoplasmofluidic Detection of Ultralow-Concentration Cytokine
| Autor: | Timothy T. Cornell, Meng Ting Chung, Katsuo Kurabayashi, Jianping Fu, Zhenhui Liu, Walker M. McHugh, Robert Nidetz, Pengyu Chen, Young Geun Park, Yujing Song |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Microfluidics Nanoparticle Bioengineering Nanotechnology Biosensing Techniques 02 engineering and technology 01 natural sciences Article Limit of Detection Lab-On-A-Chip Devices Miniaturization Humans General Materials Science Particle Size Surface plasmon resonance Electrodes Plasmon chemistry.chemical_classification Nanotubes Mechanical Engineering Biomolecule Optical Imaging 010401 analytical chemistry technology industry and agriculture Electrochemical Techniques General Chemistry Surface Plasmon Resonance 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Microelectrode chemistry Cytokines Gold Electroosmosis 0210 nano-technology Biosensor Biomarkers |
| Zdroj: | Nano Letters. 17:2374-2380 |
| ISSN: | 1530-6992 1530-6984 |
| Popis: | Label-free, nanoparticle-based plasmonic optical biosensing, combined with device miniaturization and microarray integration, has emerged as a promising approach for rapid, multiplexed biomolecular analysis. However, limited sensitivity prevents the wide use of such integrated label-free nanoplasmonic biosensors in clinical and life science applications where low-abundance biomolecule detection is needed. Here, we present a nanoplasmofluidic device integrated with microelectrodes for rapid, label-free analysis of a low-abundance cell signaling protein, detected by AC electroosmosis-enhanced localized surface plasmon resonance (ACE-LSPR) biofunctional nanoparticle imaging. The ACE-LSPR device is constructed using both bottom-up and top-down sensor fabrication methods, allowing the seamless integration of antibody-conjugated gold nanorod (AuNR) biosensor arrays with microelectrodes on the same microfluidic platform. Applying an AC voltage to microelectrodes while scanning the scattering light intensity variation of the AuNR biosensors results in significantly enhanced biosensing performance. The AC electroosmosis (ACEO) based enhancement of the biosensor performance enables rapid (5–15 min) quantification of IL-1β, a pro-inflammatory cytokine biomarker, with a sensitivity down to 158.5 fg/mL (9.1 fM) for spiked samples in PBS and 1 pg/mL (58 fM) for diluted human serum. Together with the optimized detection sensitivity and speed, our study presents the first critical step toward the application of nanoplasmonic biosensing technology to immune status monitoring guided by low-abundance cytokine measurement. |
| Databáze: | OpenAIRE |
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