Simple and rapid silver nanoparticles based antioxidant capacity assays: reactivity study for phenolic compounds
| Autor: | Manuel Sergi, Annalisa Scroccarello, Marcello Mascini, Dario Compagnone, Michele Del Carlo, Flavio Della Pelle |
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| Rok vydání: | 2018 |
| Předmět: |
Silver
Metal Nanoparticles 02 engineering and technology 01 natural sciences Silver nanoparticle Antioxidants Chemistry Techniques Analytical Analytical Chemistry Nanomaterials chemistry.chemical_compound Flavonols Reactivity (chemistry) Surface plasmon resonance chemistry.chemical_classification Chromatography ABTS Plant Extracts 010401 analytical chemistry Polyphenols Reproducibility of Results General Medicine Surface Plasmon Resonance 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Polyphenol Silver nanoparticles (AgNPs) Gold nanoparticles (AuNPs) Antioxidant capacity (AOC) assay Polyphenols Nanomaterials 0210 nano-technology Selectivity Food Science |
| Popis: | A single-step, rapid (10 min), sensitive silver nanoparticles (AgNPs) based spectrophotometric method for antioxidant capacity (AOC) assay has been developed. The assay is based on the ability of natural polyphenols to reduce Ag(I) and stabilize the produced AgNPs(0) at room temperature. Localized surface plasmon resonance (LSPR) of AgNPs at ≈420 nm is then measured. Using different conditions of pH (8.4) and temperature (45 °C) a further assay based on the production of AgNPs with selectivity for flavonols was also developed. The reactivity of the two AgNPs based assays vs. 15 polyphenols belonging to different chemical classes and 9 different samples has been studied and compared with ABTS, Folin and AuNPs based methods for AOC. The proposed assays had good reproducibility (RSD ≤ 13) and are simple, sensitive and cost effective. Moreover, used in conjunction with the classical AOC assays, can improve the information on the polyphenolic pool of food samples. |
| Databáze: | OpenAIRE |
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