Characterization of the Microbial Communities in Rumen Fluid Inoculated Reactors for the Biogas Digestion of Wheat Straw
| Autor: | Hong-Xun Zhang, Ya-Jie Zhang, Hao-Ran Zhu, Ke Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
anaerobic digestion Methanogenesis rumen fluid 030106 microbiology Geography Planning and Development ved/biology.organism_classification_rank.species lcsh:TJ807-830 lcsh:Renewable energy sources Management Monitoring Policy and Law Microbiology 03 medical and health sciences Rumen lignocellulose inoculum concentration methanogenesis Biogas Food science lcsh:Environmental sciences lcsh:GE1-350 biology Renewable Energy Sustainability and the Environment ved/biology Ruminococcus lcsh:Environmental effects of industries and plants Methanosarcina Straw biology.organism_classification equipment and supplies Anaerobic digestion lcsh:TD194-195 Methanosarcina barkeri |
| Zdroj: | Sustainability, Vol 9, Iss 2, p 243 (2017) Sustainability; Volume 9; Issue 2; Pages: 243 |
| ISSN: | 2071-1050 |
| Popis: | The present study investigated the effect of rumen fluid (RF) concentration on the methane production through anaerobic digestion of wheat straw in batch mode, and compared the microbial communities in RF and RF inoculated reactors by 16S rRNA genes sequencing. Six levels of RF concentration including 1%, 5%, 10%, 15%, 20% and 25% (v/v) were used in reactors R1, R5, R10, R15, R20 and R25 respectively. The results revealed that lower than or equal to 5% RF concentrations resulted in reactor acidification and low methane production. The highest methane yield of 106 mL CH4 g VS−1 was achieved in R10, whereas higher RF concentrations than 10% could not improve the methane production significantly. Methanosarcina barkeri was abundant in the well-working reactors, and Methanobacterium was dominant in the poor-working reactors, implying the archaeal communities in reactors had changed greatly from the Methanobrevibacter-dominated RF. Although the relative abundance of Clostridium and Ruminococcus were greatly different between RF and reactors, the Bacteroidetes and Firmicutes communities were dominant in all the tested samples. The results indicated that the in vitro anaerobic conditions had altered the rumen methanogenic communities significantly and the facultative acetoclastic Methanosarcina was important for the methane production in the RF seeded reactors. |
| Databáze: | OpenAIRE |
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