Inhibition of Epstein–Barr virus (EBV)-specific CD8+ cytotoxic T lymphocyte (CTL) activity by soluble HLA class I in vitro
| Autor: | S Kato, Namid Munkhtuvshin, Nobuyuki Kanai, J. Chargui, Tomomitsu Hotta, Batmunkh Munkhbat, Naokatsu Morita, B Gansuvd, Masao Hagihara, Kimiyoshi Tsuji |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Herpesvirus 4
Human Immunology chemical and pharmacologic phenomena Apoptosis Human leukocyte antigen Biology In Vitro Techniques medicine.disease_cause Cell Line HLA-B7 Antigen Immune system HLA Antigens medicine Immunology and Allergy Cytotoxic T cell Humans B-Lymphocytes Histocompatibility Antigens Class I hemic and immune systems Immunity to Infection T lymphocyte Cell Transformation Viral Epstein–Barr virus In vitro Recombinant Proteins CTL CD8 T-Lymphocytes Cytotoxic |
| Popis: | SUMMARYIn the present study, the effects of soluble HLA (sHLA) class I molecules against EBV-specific CTL were examined. Two different sources of sHLA class I, either bioengineered spliced form of HLA-B7 (sB7) or natural production from EBV-transformed B cells (natural sHLA), were added during the induction of CTL or incubated with MHC-restricted CD8+ CTL, which were selected by immunobeads just before testing for their cytotoxic activity. Both sB7 and natural sHLA class I blocked the generation of CD8+ CTL and also inhibited the cytotoxic activity of established CTL in a dose-dependent manner. In both ways, natural sHLA class I was effective in 10-fold lower concentrations compared with sB7. The inhibitory effect did not require a sharing of the HLA allotypes between sHLA and the CTL. CTL, after being treated with sHLA, underwent apoptosis, which was considered here as the main mechanism. |
| Databáze: | OpenAIRE |
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