Comparison of the effects of osmotic pump implantation with subcutaneous injection for administration of drugs after total body irradiation in mice
| Autor: | Katherine Peachee, Akira Isaac, W. Bradley Rittase, Milan Rusnak, Regina M. Day, Elizabeth A McCart, Amory L Koch, Cara H. Olsen, Aviva J. Symes |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_treatment
Injections Subcutaneous Osmotic pump 03 medical and health sciences Subcutaneous injection Mice 0302 clinical medicine Weight loss medicine Animals Irradiation Saline 030304 developmental biology 0303 health sciences General Veterinary business.industry Acute Radiation Syndrome Infusion Pumps Implantable Total body irradiation Mice Inbred C57BL Disease Models Animal 030220 oncology & carcinogenesis Anesthesia Drug delivery Animal Science and Zoology Female medicine.symptom business Whole-Body Irradiation |
| Zdroj: | Laboratory animals. 55(2) |
| ISSN: | 1758-1117 |
| Popis: | The increasing potential for radiation exposure from nuclear accidents or terrorist activities has intensified the need to develop pharmacologic countermeasures against injury from total body irradiation (TBI). Many initial experiments to develop and test these countermeasures utilize murine irradiation models. Yet, the route of drug administration can alter the response to irradiation injury. Studies have demonstrated that cutaneous injuries can exacerbate damage from radiation, and thus surgical implantation of osmotic pumps for drug delivery could adversely affect the survival of mice following TBI. However, daily handling and injections to administer drugs could also have negative consequences. This study compared the effects of subcutaneous needlesticks with surgical implantation of osmotic pumps on morbidity and mortality in a murine model of hematopoietic acute radiation syndrome (H-ARS). C57BL/6 mice were sham irradiated or exposed to a single dose of 7.7 Gy60Co TBI. Mice were implanted with osmotic pumps containing sterile saline seven days prior to irradiation or received needlesticks for 14 days following irradiation or received no treatment. All irradiated groups exhibited weight loss. Fewer mice with osmotic pumps survived to 30 days post irradiation (37.5%) than mice receiving needlesticks or no treatment (70% and 80%, respectively), although this difference was not statistically significant. However, mice implanted with the pump lost significantly more weight than mice that received needlesticks or no treatment. These data suggest that surgical implantation of a drug-delivery device can adversely affect the outcome in a murine model of H-ARS. |
| Databáze: | OpenAIRE |
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