Development of multi-response optimization and quadratic calibration curve for determination of ten pesticides in complex sample matrices using QuEChERS dispersive liquid-liquid microextraction followed by gas chromatography
| Autor: | Parvaneh Balsini, Hadi Parastar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Gas Central composite design Calibration (statistics) Calibration curve Liquid Phase Microextraction Filtration and Separation Food Contamination 02 engineering and technology Quechers 01 natural sciences Analytical Chemistry law.invention law Limit of Detection media_common.cataloged_instance Flame ionization detector Animals European union Pesticides media_common Mathematics Detection limit Chromatography 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Milk Calibration Cattle Gas chromatography 0210 nano-technology Water Pollutants Chemical |
| Zdroj: | Journal of separation scienceREFERENCES. 42(23) |
| ISSN: | 1615-9314 |
| Popis: | In this study, QuEChERS combined with dispersive liquid-liquid microextraction is developed for extraction of ten pesticides in complex sample matrices of water and milk. In this regard, effective factors of proposed extraction technique combined with gas chromatography with flame ionization detector were designed, modeled, and optimized using central composite design, multiple linear regression, and Nelder-Mead simplex optimization. Later, univariate calibration model for ten pesticides was developed in concentration range of 0.5-100 ng/mL. Surprisingly, quadratic calibration behavior was observed for some of the pesticides. In this regard, Mandel's test was used for evaluating linearity and types of calibration equation. Finally, four pesticides followed linear calibration curve with sensitivity (0.23-0.66 mL/ng), analytical sensitivity (0.20-0.32), regression coefficient (0.988-0.995), limit of detection (0.39-1.83 ng/mL), and limit of quantitation (1.30-6.10 ng/mL) and six of them followed quadratic calibration curve with sensitivity (0.18-0.93 mL/ng), analytical sensitivity (0.25-0.86), regression coefficient (0.944-0.999), limit of detection (0.59-1.92 ng/mL), and limit of quantitation (1.96-6.40 ng/mL). The calculated limits of detection were below the maximum residue limits according to European Union pesticides database of European Commission. Finally, the proposed analytical method was used for determination of ten pesticides in water and milk samples. |
| Databáze: | OpenAIRE |
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