The alternative complement pathway aids in vascular regression during the early stages of a murine model of proliferative retinopathy
| Autor: | Alexandra Castillejos, Clifford Kim, Magali Saint-Geniez, Daniel Diaz-Aguilar, Kaylee E. Smith, Kip M. Connor |
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| Rok vydání: | 2015 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Complement Pathway Alternative innate immune system CD59 Hyperoxia Retinal Neovascularization Biology Vegf Biochemistry Vascular Regression Complement factor B Retina Andrology Neovascularization Research Communication Mice 03 medical and health sciences chemistry.chemical_compound Genetics medicine Animals Protein Isoforms Cd55 Molecular Biology Cd59 Neovascularization Pathologic Vitreoretinopathy Proliferative Retinal Vessels Retinal OIR medicine.disease Mice Inbred C57BL Oxygen Disease Models Animal Vascular endothelial growth factor A 030104 developmental biology Animals Newborn chemistry factor b Immunology Alternative complement pathway medicine.symptom Biotechnology Retinopathy |
| Zdroj: | The FASEB Journal |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | Proliferative retinopathic diseases often progress in 2 phases: initial regression of retinal vasculature (phase 1) followed by subsequent neovascularization (NV) (phase 2). The immune system has been shown to aid in vascular pruning in such retinopathies; however, little is known about the role of the alternative complement pathway in the initial vascular regression phase. Using a mouse model of oxygen-induced retinopathy (OIR), we observed that alternative complement pathway–deficient mice (Fb−/−) exhibited a mild decrease in vascular loss at postnatal day (P)8 compared with age- and strain-matched controls (P = 0.035). Laser capture microdissection was used to isolate the retinal blood vessels. Expression of the complement inhibitors Cd55 and Cd59 was significantly decreased in blood vessels isolated from hyperoxic retinas compared with those from normoxic control mice. Vegf expression was measured at P8 and found to be significantly lower in OIR mice than in normoxic control mice (P = 0.0048). Further examination of specific Vegf isoform expression revealed a significant decrease in Vegf120 (P = 0.00032) and Vegf188 (P = 0.0092). In conjunction with the major modulating effects of Vegf during early retinal vascular development, our data suggest a modest involvement of the alternative complement pathway in targeting vessels for regression in the initial vaso-obliteration stage of OIR.—Kim, C., Smith, K. E., Castillejos, A., Diaz-Aguilar, D., Saint-Geniez, M., Connor, K. M. The alternative complement pathway aids in vascular regression during the early stages of a murine model of proliferative retinopathy. |
| Databáze: | OpenAIRE |
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