Diversity of Francisella Species in Environmental Samples from Martha’s Vineyard, Massachusetts
| Autor: | Sam R. Telford, Zenda L. Berrada |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Soil Science medicine.disease_cause Polymerase Chain Reaction complex mixtures Microbiology law.invention Tularemia 03 medical and health sciences Phylogenetics law RNA Ribosomal 16S Zoonoses Environmental Microbiology medicine Animals Francisella novicida Francisella Phylogeny Ecology Evolution Behavior and Systematics Polymerase chain reaction 030304 developmental biology 0303 health sciences Ecology biology 030306 microbiology Outbreak Biodiversity Sequence Analysis DNA respiratory system Ribosomal RNA bacterial infections and mycoses 16S ribosomal RNA biology.organism_classification medicine.disease Succinate Dehydrogenase Massachusetts DNA Transposable Elements bacteria Water Microbiology Bacterial Outer Membrane Proteins |
| Zdroj: | Microbial Ecology |
| ISSN: | 1432-184X 0095-3628 |
| Popis: | We determined whether Francisella spp. are present in water, sediment, and soil from an active tularemia natural focus on Martha's Vineyard, Massachusetts, during a multiyear outbreak of pneumonic tularemia. Environmental samples were tested by polymerase chain reaction (PCR) targeting Francisella species 16S rRNA gene and succinate dehydrogenase A (sdhA) sequences; evidence of the agent of tularemia was sought by amplification of Francisella tularensis-specific sequences for the insertion element ISFTu2, 17-kDa protein gene tul4, and the 43-kDa outer membrane protein gene fopA. Evidence of F. tularensis subsp. tularensis, the causative agent of the human infections in this outbreak, was not detected from environmental samples despite its active transmission among ticks and animals in the sampling site. Francisella philomiragia was frequently detected from a brackish-water pond using Francisella species PCR targets, and subsequently F. philomiragia was isolated from an individual brackish-water sample. Distinct Francisella sp. sequences that are closely related to F. tularensis and Francisella novicida were detected from samples collected from the brackish-water pond. We conclude that diverse Francisella spp. are present in the environment where human cases of pneumonic tularemia occur. |
| Databáze: | OpenAIRE |
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