Toxicity and inflammatory response in Swiss albino mice after intraperitoneal and oral administration of polyurethane nanoparticles
| Autor: | Adny Henrique Silva, Andrew Owen, Neill J. Liptrott, Philip Martin, Fabíola Branco Filippin-Monteiro, Claudriana Locatelli, André A. Pasa, Tânia Beatriz Creczynski-Pasa, Evelize M. Nazari, Cláudia A.C. Albuquerque, Betina G. Zanetti-Ramos, Luana C. Benetti |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Polyurethanes Administration Oral Adipose tissue Inflammation 02 engineering and technology Pharmacology Toxicology Proinflammatory cytokine Mice 03 medical and health sciences Oral administration In vivo medicine Animals Humans Interleukin-6 Tumor Necrosis Factor-alpha business.industry General Medicine 021001 nanoscience & nanotechnology 030104 developmental biology Toxicity Nanoparticles Liver function medicine.symptom 0210 nano-technology business Injections Intraperitoneal Ex vivo |
| Zdroj: | Toxicology Letters. 246:17-27 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2016.01.018 |
| Popis: | In this work in vivo experiments were conducted in order to characterize the biocompatibility of polyurethane nanoparticles (PU-NPs) after intraperitoneal (i.p.) and oral administration. Additionally, ex vivo assays were performed to assess human blood compatibility as well as in vitro assays to assess protein binding. Our results indicated that administration of three different concentrations of PU-NPs induced a significant increase in visceral fat accumulation after oral dosing. In addition, fat tissue of mice intraperitoneally treated with the highest concentration of nanoparticles showed diffuse mononuclear inflammatory infiltrate in the fat tissue. Histopathological assessment showed inflammatory infiltrate and hepatocyte vacuolization in the liver, inflammatory infiltration and vascular congestion in the lung and glomerular necrosis in the kidney. Hepatic enzymes related with liver function were significantly increased in both groups of mice treated with PU-NPs. The PU-NPs did not affect the human blood cells number as well as coagulation time but showed a susceptibility to bind in proteins commonly found in the blood stream. In addition, increased amounts of pro inflammatory cytokines in vivo, as well as ex vivo in human cells were observed. Further studies to establish the consequences of long-term exposure to PU-NPs are warranted. |
| Databáze: | OpenAIRE |
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