Robust Saliva-Based RNA Extraction-Free One-Step Nucleic Acid Amplification Test for Mass SARS-CoV-2 Monitoring

Autor: Roman Jerala, Petra Sušjan, T. Demšar, Gabriele Turel, Viktorija Tomič, Žiga Jensterle, Mihaela Zidarn, Tatjana Lejko-Zupanc, Alenka Šmid, Mojca Benčina, Dunja Urbančič, Eva Rajh, Katarina Prosenc Trilar, Irena Mlinarič-Raščan, Polona Kogovšek, Mojca Milavec, Arne Praznik, Tina Šket
Rok vydání: 2021
Předmět:
Saliva
passive drool
real-time polymerase chain reaction
oral cavity swab
Pharmaceutical Science
Analytical Chemistry
COVID-19 Testing
QD241-441
Drug Discovery
Mass Screening
Medicine
Verižna reakcija s polimerazo v realnem času
verižna reakcija s polimerazo v realnem času
Subclinical infection
COVID-19 serological testing
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
Real-time polymerase chain reaction
Covid-19
Chemistry (miscellaneous)
RNA
Viral
Molecular Medicine
RNA extraction
medicine.symptom
Nucleic Acid Amplification Techniques
bris ustne sluznice
Loop-mediated isothermal amplification
Slina
Sensitivity and Specificity
Asymptomatic
Article
slina
Specimen Handling
serološko testiranje za COVID-19
LAMP
Saliva testing
udc:616.9
Humans
pooling
Physical and Theoretical Chemistry
Mass screening
oral cavity swab
passive drool
pooling
saliva
Serološko testiranje za COVID-19
business.industry
RT-qPCR
Organic Chemistry
COVID-19
Nucleic acid amplification technique
Virology
združeni vzorci
RNA
business
bris ustne sluznice
združeni vzorci
Zdroj: Molecules
Molecules, Vol 26, Iss 6617, p 6617 (2021)
Molecules, str. 1-19, Vol. 26, iss. 21, art. 6617, 2021 : Ilustr.
COBISS-ID: 18462981
Molecules, vol. 26, no. 21, pp. 1-19, 2021.
Volume 26
Issue 21
Molecules, vol. 26, no. 21, 6617, 2021.
ISSN: 1420-3049
Popis: Early diagnosis with rapid detection of the virus plays a key role in preventing the spread of infection and in treating patients effectively. In order to address the need for a straightforward detection of SARS-CoV-2 infection and assessment of viral spread, we developed rapid, sensitive, extraction-free one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) tests for detecting SARS-CoV-2 in saliva. We analyzed over 700 matched pairs of saliva and nasopharyngeal swab (NSB) specimens from asymptomatic and symptomatic individuals. Saliva, as either an oral cavity swab or passive drool, was collected in an RNA stabilization buffer. The stabilized saliva specimens were heat-treated and directly analyzed without RNA extraction. The diagnostic sensitivity of saliva-based RT-qPCR was at least 95% in individuals with subclinical infection and outperformed RT-LAMP, which had at least 70% sensitivity when compared to NSBs analyzed with a clinical RT-qPCR test. The diagnostic sensitivity for passive drool saliva was higher than that of oral cavity swab specimens (95% and 87%, respectively). A rapid, sensitive one-step extraction-free RT-qPCR test for detecting SARS-CoV-2 in passive drool saliva is operationally simple and can be easily implemented using existing testing sites, thus allowing high-throughput, rapid, and repeated testing of large populations. Furthermore, saliva testing is adequate to detect individuals in an asymptomatic screening program and can help improve voluntary screening compliance for those individuals averse to various forms of nasal collections. Nasl. z nasl. zaslona. Soavtorji: Tina Šket, Arne Praznik, Petra Sušjan, Alenka Šmid, Dunja Urbančič, Irena Mlinarič-Raščan, Polona Kogovšek, Tina Demšar, Mojca Milavec, Katarina Prosenc Trilar, Žiga Jensterle, Mihaela Zidarn, Viktorija Tomič, Gabriele Turel, Tatjana Lejko-Zupanc, Roman Jerala, Mojca Benčina. Opis vira z dne 5. 11. 2021. Eva Rajh in Tina Šket sta enakovredni prvi avtorici. Št. članka: 6617. Bibliografija: str. 17-19. Abstract. ARRS ARRS ARRS ARRS ARRS Ministry of Education, Science, and Sport (MIZŠ) and the European Regional Development Fund, RI-SI-EATRIS
Databáze: OpenAIRE
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