Alterations of Mitochondrial Protein Assembly and Jasmonic Acid Biosynthesis Pathway in Honglian (HL)-type Cytoplasmic Male Sterility Rice
| Autor: | Yu-Qi Feng, Lin Guo, Yanxiao Ji, Shaoqing Li, Yingguo Zhu, Yun-Qing Huang, Gai Liu, Jun Hu, Han Tian |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Plant Infertility
Plant Biology Cyclopentanes Chimeric gene Biology Mitochondrion Proteomics Biochemistry Mitochondrial Proteins chemistry.chemical_compound Microspore health services administration Oxylipins Molecular Biology Gene health care economics and organizations Plant Proteins Jasmonic acid Cytoplasmic male sterility Oryza Cell Biology Proton-Translocating ATPases chemistry Proteome Pollen |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m112.382549 |
| Popis: | It has been suggested that the mitochondrial chimeric gene orfH79 is the cause for abortion of microspores in Honglian cytoplasmic male sterile rice, yet little is known regarding its mechanism of action. In this study, we used a mass spectrometry-based quantitative proteomics strategy to compare the mitochondrial proteome between the sterile line Yuetai A and its fertile near-isogenic line Yuetai B. We discovered a reduced quantity of specific proteins in mitochondrial complexes in Yuetai A compared with Yuetai B, indicating a defect in mitochondrial complex assembly in the sterile line. Western blotting showed that ORFH79 protein and ATP1 protein, an F(1) sector component of complex V, are both associated with large protein complexes of similar size. Respiratory complex activity assays and transmission electron microscopy revealed functional and morphological defects in the mitochondria of Yuetai A when compared with Yuetai B. In addition, we identified one sex determination TASSELSEED2-like protein increased in Yuetai A, leading to the discovery of an aberrant variation of the jasmonic acid pathway during the development of microspores. |
| Databáze: | OpenAIRE |
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