Differential fluorochromasia of human lymphocytes as measured by flow cytometry
| Autor: | L S Cram, M E Wilder |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Fluorescein diacetate
Histology Somatic cell T cell T-Lymphocytes Biology Fluorescence Flow cytometry chemistry.chemical_compound medicine Fluorescein B cell B-Lymphocytes medicine.diagnostic_test Staining and Labeling Differential staining Temperature Fluoresceins Molecular biology Immune Adherence Reaction medicine.anatomical_structure Spectrometry Fluorescence chemistry Biochemistry Anatomy |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 25(7) |
| ISSN: | 0022-1554 |
| Popis: | Peripheral human lymphocytes reacted with fluorescein diacetate and analyzed by flow cytometry produced a bimodal fluorescence distribution that was shown to be attributable to the differential staining of T and B lymphocytes. Lymphocytes were fractionated into rosetting (T cell) and nonrosetting (B cell) populations. Both subfractions were reacted with fluorescein diacetate and analyzed by flow cytometry. The rosetting fraction was more fluorescent than the nonrosetting fraction, and the analysis of an appropriate mixture of the subfractionated populations produced a fluorescence distribution very similar to that obtained with unfractionated lymphocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|