| Autor: |
Martino, Simona Di, Luca, Gabriele De, Grassi, Ludovica, Federici, Giulia, Alfonsi, Romina, Signore, Michele, Addario, Antonio, Salvo, Laura De, Francescangeli, Federica, Sanchez, Massimo, Tirelli, Valentina, Muto, Giovanni, Sperduti, Isabella, Steno Sentinelli, Costantini, Manuela, Pasquini, Luca, Milella, Michele, Haoui, Mustapha, Simone, Giuseppe, Gallucci, Michele, Maria, Ruggero De, Bonci, Désirée |
| Rok vydání: |
2018 |
| DOI: |
10.6084/m9.figshare.7052795.v1 |
| Popis: |
Figure S6. (A) Freshly dissociated tissues maintained for one week in serum-free stem cell-isolating medium supplemented with Epidermal Growth Factor (EGF), basic Fibroblast Growth Factor (b-FGF), DMEM (Dulbecco Modified Eagle Medium), or Glutamine and FBS (Fetal Bovine Serum) supplemented medium, and analyzed by cytofluorimetric analysis. CD45 (PE-Cy7), CD146 (PE), CD44 (H450-Pacific Blue) and EpCAM (FITC) antigens were analyzed. TOPRO3 was used for gating vital cells. (B) The histograms report growth rate fold change of cells described in A, 4 and 10 days after sorting. Control represents (red dashed line) value = 1 i.e. reference relative count at sorting and plating day. Mean of three independent experiments is reported. Values are mean ± s.d (C) Colony forming assay of EpCAM+/CD146+/CD44+ and triple negative sorted cells and non-sorted population maintained in culture one week in stem serum free medium (D) Mean colony size of EpCAM+/CD146+/CD44+ and triple negative sorted cells and non-sorted population maintained in culture one week in stem serum free medium. Mean of three independent experiments is reported. Values are mean ± s.d. (PDF 231 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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