Phenolic acridine orange fluorescent stain for mycobacteria
Autor: | C K Wallis, R W Smithwick, M A Karlix, M R Bigbie, R B Ferguson |
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Jazyk: | angličtina |
Rok vydání: | 1995 |
Předmět: |
Microbiology (medical)
Auramine-rhodamine stain Staining and Labeling Differential staining Acridine orange Biology Molecular biology Stain Benzophenoneidum Acridine Orange Staining Mycobacterium chemistry.chemical_compound chemistry Microscopy Fluorescence Species Specificity Acridine Field stain Acids Research Article Fluorescent Dyes |
Popis: | A new fluorescence acid-fast staining method with acridine orange as the specific stain is presented. Only two reagents are required: the acridine orange-specific stain and a destaining-counterstaining reagent. Compared with auramine fluorescence acid-fast staining, there was less nonspecific staining of non-acid-fast debris which fluoresced a pale green contrasting color to provide a background in which to search for the red-to-orange fluorescing acid-fast bacilli. The results of the study indicate that the acridine orange method is superior to the auramine method in detecting acid-fast bacilli in specimen smears. |
Databáze: | OpenAIRE |
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