Representative Bacillus sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation
| Autor: | Margarita Aguilera, Ana López-Moreno, Alfonso Torres-Sánchez, Antonio Suárez, Inmaculada Acuña |
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| Přispěvatelé: | [López-Moreno,A, Torres-Sánchez,A, Aguilera,M] Department of Microbiology, Faculty of Pharmacy, University of Granada, Granada, Spain. [López-Moreno,A, Acuña,I, Suárez,A, Aguilera,M] Instituto de Nutrición y Tecnología de los Alimentos, INYTA-Granada, Granada, Spain. [Acuña,I, Suárez,A] Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Granada, Granada, Spain. [Aguilera,M] IBS: Instituto de Investigación Biosanitaria ibs., Granada, Spain., This research was partially funded by grant number GP/EFSA/ENCO/380 2018/03/G04:OBEMIRISK: Knowledge platform for assessing the risk of Bisphenols on gut microbiota and its role in obesogenic phenotype: looking for biomarkers. This research was also funded by FEDER Infrastructure: IE_2019-198. The APC was funded by EIN-2019-103082. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Enzimas Microorganism Bacillus Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::RNA::RNA Ribosomal::RNA Ribosomal 16S [Medical Subject Headings] Bisphenols 010501 environmental sciences Gut flora Organisms::Bacteria::Gram-Positive Bacteria::Bacillales::Bacillaceae::Bacillus [Medical Subject Headings] 01 natural sciences lcsh:Chemistry Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] chemistry.chemical_compound Polisacáridos RNA Ribosomal 16S Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Microbial::Genome Bacterial [Medical Subject Headings] Biology (General) lcsh:QH301-705.5 Phylogeny Spectroscopy Biología molecular biology Microbiota Human microbiome General Medicine Isolation (microbiology) Anti-Bacterial Agents Computer Science Applications Enzymes Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Microbiological Techniques::Microbial Sensitivity Tests [Medical Subject Headings] Chemistry Biodegradation Environmental Biochemistry Phenomena and Processes::Chemical Phenomena::Chemical Processes::Biochemical Processes::Signal Transduction [Medical Subject Headings] Signal Transduction Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Therapeutic Uses::Anti-Infective Agents::Anti-Bacterial Agents [Medical Subject Headings] endocrine system Bacillus amyloliquefaciens QH301-705.5 PHA enzymes Polihidroxialcanoatos Microbial Sensitivity Tests Article Catalysis Inorganic Chemistry 03 medical and health sciences Phenols Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons Cyclic::Hydrocarbons Aromatic::Benzene Derivatives::Phenols [Medical Subject Headings] medicine Humans Benzhydryl Compounds Physical and Theoretical Chemistry human microbiota Molecular Biology QD1-999 0105 earth and related environmental sciences Molecular pathways Organic Chemistry Health Care::Environment and Public Health::Public Health::Environmental Pollution::Environmental Remediation::Biodegradation Environmental [Medical Subject Headings] Chemicals and Drugs::Organic Chemicals::Hydrocarbons::Hydrocarbons Cyclic::Hydrocarbons Aromatic::Benzene Derivatives::Benzhydryl Compounds [Medical Subject Headings] biology.organism_classification medicine.disease Gastrointestinal Microbiome 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry bisphenols Phenomena and Processes::Genetic Phenomena::Phylogeny [Medical Subject Headings] Human microbiota EPS Xenobiotic Dysbiosis molecular pathways Genome Bacterial |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname International Journal of Molecular Sciences, Vol 22, Iss 4952, p 4952 (2021) International Journal of Molecular Sciences Volume 22 Issue 9 Issue 5 International Journal of Molecular Sciences, Vol 22, Iss 2425, p 2425 (2021) Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) |
| ISSN: | 2019-1030 |
| Popis: | Human gut microbiota harbors numerous microbial species with molecular enzymatic potential that impact on the eubiosis/dysbiosis and health/disease balances. Microbiota species isolation and description of their specific molecular features remain largely unexplored. In the present study, we focused on the cultivation and selection of species able to tolerate or biodegrade the endocrine disruptor bisphenol A (BPA), a xenobiotic extensively found in food plastic containers. Chemical xenobiotic addition methods for the directed isolation, culturing, Whole Genome Sequencing (WGS), phylogenomic identification, and specific gene-encoding searches have been applied to isolate microorganisms, assess their BPA metabolization potential, and describe encoded catabolic pathways. BPA-tolerant strains were isolated from 30% of infant fecal microbial culture libraries analyzed. Most isolated strains were phylogenetically related to the operational taxonomic group Bacillus amyloliquefaciens spp. Importantly, WGS analysis of microbial representative strain, Bacillus sp. AM1 identified the four complete molecular pathways involved on BPA degradation indicating its versatility and high potential to degrade BPA. Pathways for Exopolysaccharide (EPS) and Polyhydroxyalkanates (PHA) biopolymer synthesis were also identified and phenotypically confirmed by transmission electronic microscopy (TEM). These microbial biopolymers could generally contribute to capture and/or deposit xenobiotics. GP/EFSA/ENCO/380 2018/03/G04: OBEMIRISK: Knowledge platform for assessing the risk of Bisphenols on gut microbiota and its role in obesogenic phenotype: looking for biomarkers FEDERInfrastructure: IE_2019-198 APC was funded by EIN-2019-103082 |
| Databáze: | OpenAIRE |
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