Functional implication of archaeal homologues of human RNase P protein pair Pop5 and Rpp30
| Autor: | Masato Hamasaki, Takashi Nakashima, Toshifumi Ueda, Makoto Kimura, Kohsuke Hazeyama, Yoshimitsu Kakuta, Fumihiko Iwasaki |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
RNase P Stereochemistry Archaeal Proteins RNA Archaeal Biology Biochemistry RNase PH Autoantigens Ribonuclease P 03 medical and health sciences Pyrococcus horikoshii Pyrococcus Tetramer Humans Molecular Biology Base Sequence Oligonucleotide Regular Papers General Medicine Surface Plasmon Resonance biology.organism_classification Heterotetramer Protein Structure Tertiary RNase MRP Kinetics Protein Subunits 030104 developmental biology Molecular Chaperones |
| Zdroj: | Journal of biochemistry. 159(1) |
| ISSN: | 1756-2651 |
| Popis: | PhoPop5 and PhoRpp30 in the hyperthermophilic archaeon Pyrococcus horikoshii, homologues of human ribonuclease P (RNase P) proteins hPop5 and Rpp30, respectively, fold into a heterotetramer [PhoRpp30-(PhoPop5)2-PhoRpp30], which plays a crucial role in the activation of RNase P RNA (PhopRNA). Here, we examined the functional implication of PhoPop5 and PhoRpp30 in the tetramer. Surface plasmon resonance (SPR) analysis revealed that the tetramer strongly interacts with an oligonucleotide including the nucleotide sequence of a stem-loop SL3 in PhopRNA. In contrast, PhoPop5 had markedly reduced affinity to SL3, whereas PhoRpp30 had little affinity to SL3. SPR studies of PhoPop5 mutants further revealed that the C-terminal helix (α4) in PhoPop5 functions as a molecular recognition element for SL3. Moreover, gel filtration indicated that PhoRpp30 exists as a monomer, whereas PhoPop5 is an oligomer in solution, suggesting that PhoRpp30 assists PhoPop5 in attaining a functionally active conformation by shielding hydrophobic surfaces of PhoPop5. These results, together with available data, allow us to generate a structural and mechanistic model for the PhopRNA activation by PhoPop5 and PhoRpp30, in which the two C-terminal helices (α4) of PhoPop5 in the tetramer whose formation is assisted by PhoRpp30 act as binding elements and bridge SL3 and SL16 in PhopRNA. |
| Databáze: | OpenAIRE |
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