Nucleotide sequence analysis of the structural genes for Shiga-like toxin I encoded by bacteriophage 933J from Escherichia coli
| Autor: | John W. Newland, Randall K. Holmes, Matthew P. Jackson, Alison D. O'Brien |
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| Rok vydání: | 1987 |
| Předmět: |
Genes
Viral genetic structures Macromolecular Substances Base pair Bacterial Toxins Molecular Sequence Data Restriction Mapping Biology Shiga Toxin 1 medicine.disease_cause Coliphages Microbiology chemistry.chemical_compound Shiga-like toxin Escherichia coli medicine Amino Acid Sequence Peptide sequence Gene Genetics Base Sequence Structural gene Nucleic acid sequence Open reading frame Infectious Diseases Genes chemistry |
| Zdroj: | Microbial Pathogenesis. 2:147-153 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/0882-4010(87)90106-9 |
| Popis: | A nucleotide sequence analysis was performed on the Shiga-like toxin I genes previously cloned from the Escherichia coli bacteriophage 933J. Two structural genes designated slt-I A and slt-I B were found to be oriented on a single transcriptional unit with slt-I A preceding slt-I B. A 12 base pair gap separated the in-phase open reading frames of slt-I A and slt-I B. Putative ribosome binding sites were identified 5' to both the slt-I A and slt-I B genes. Translation of the SLT-I nucleotide sequence revealed that both the A and B subunits were synthesized with signal peptides. The molecular weight calculated for the mature A subunit was 32,211 while the molecular weight of the mature B subunit was 7690. |
| Databáze: | OpenAIRE |
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