Simultaneous determination of amoxicillin and chloramphenicol and their drug interaction study by the validated UPLC method

Autor:	Rezaul Karim, Mohammad Nasir Uddin, H. R. Bhuiyan, Swapon Kumar Shill, Suman Das, Shakhawat Hasan Khan
Rok vydání:	2016
Předmět:	Chromatography UPLC method Correlation coefficient Calibration curve Chemistry Drug interaction Chloramphenicol 010401 analytical chemistry Antibiotic Analytical chemistry Amoxicillin 02 engineering and technology Standard solution 021001 nanoscience & nanotechnology 01 natural sciences High-performance liquid chromatography 0104 chemical sciences chemistry.chemical_compound medicine Particle size 0210 nano-technology Ammonium acetate medicine.drug
Zdroj:	Journal of Taibah University for Science. 10(5):755-765
ISSN:	1658-3655
DOI:	10.1016/j.jtusci.2015.11.005
Popis:	A new UPLC method was developed for the simultaneous estimation of amoxicillin and chloramphenicol in pharmaceutical formulations. Drugs were resolved on Shim-Pack XR-ODS (2 mm i.d. × 50 mm, particle size 2.2 μm) reverse-phase column, utilizing a mobile phase of methanol:0.0025 M ammonium acetate in the initial ratio 40:60 (v/v) at a gradient program. The mobile phase was pumped at a flow rate of 0.25 mL/min with detection at 220 nm. 10 μL volume of sample was injected by the auto sampler. Separation was completed within 2.72 ± 0.03 min. Calibration curves were linear with correlation coefficient 0.999 over a studied concentration range of 10 μg mL−1 for both drugs. Method was found to be reproducible with relative standard deviation (RSD) for intra- and interday precision to be
Databáze:	OpenAIRE
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