A natural oil increases specific anti-OVA IgG levels and induces a cellular immune response combined with aluminum hydroxide
| Autor: | Ivana Dalila Montironi, Laura Noelia Cariddi, Elina Beatriz Reinoso, Federico Nahuel Ruiz Moreno, Noelia Anahí Campra, Belkys A. Maletto, Jose Maria Raviolo |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Cellular immunity Ovalbumin medicine.medical_treatment Immunology Aluminum Hydroxide Pharmacology 03 medical and health sciences Mice 0302 clinical medicine Immune system Antigen Adjuvants Immunologic medicine Oils Volatile Immunology and Allergy Animals Plant Oils Minthostachys verticillata Immunity Cellular Mice Inbred BALB C Innate immune system Lamiaceae biology Cell Biology biology.organism_classification 030104 developmental biology 030220 oncology & carcinogenesis Immunoglobulin G Humoral immunity biology.protein Antibody Adjuvant |
| Zdroj: | Journal of leukocyte biologyREFERENCES. 109(1) |
| ISSN: | 1938-3673 |
| Popis: | The efficacy of certain vaccines is improved by the use of adjuvants. Nowadays, the development of new, effective, and safe adjuvants that stimulate the innate immune response is researched. In this context, medicinal plants appear as a suitable alternative. Minthostachys verticillata essential oil (EO) has demonstrated the ability to modulate mechanisms of the innate immune response. Thus, the present work aimed to evaluate the EO adjuvant effect on humoral and cellular immunity, coadministered with OVA as antigen. The chemical analysis of EO by gas chromatography–mass spectrometry revealed a predominant pulegone–menthone chemotype. EO (1.25, 2.5, or 5.0 mg/ml) did not alter the viability of murine fibroblasts (3T3 cell line) neither showed signs of toxicity in Balb/c mice inoculated subcutaneously. The serum of mice immunized with OVA + EO showed increased levels of anti-OVA-specific antibodies of IgG1 subclass compared with the mice immunized with OVA alone revealing an adjuvant effect of EO. The delayed type hypersensitivity showed that the combination OVA + Al(OH)3 + EO was the best to induce a cellular immune response that extended until 48 h postinjection of OVA. M. verticillata EO appears as a new, safe, and effective adjuvant, which should continue to be studied for their possible future incorporation into vaccine formulations. |
| Databáze: | OpenAIRE |
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