Naturally-occurring, osmo-remedial variants of Escherichia coli
| Autor: | C. M. Kunin, W E Maher, H. H. Tong |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Niacinamide Microbiology (medical) Fimbria Bacteremia Sodium Chloride medicine.disease_cause Microbiology Agar plate chemistry.chemical_compound Escherichia coli medicine Humans Mannitol Escherichia coli Infections biology Osmotic concentration Nicotinamide Osmolar Concentration General Medicine Penicillinase biology.organism_classification Enterobacteriaceae Culture Media chemistry Biochemistry Urinary Tract Infections Ampicillin Electrophoresis Polyacrylamide Gel Female Bacteria Bacterial Outer Membrane Proteins medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 38:216-221 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/00222615-38-3-216 |
| Popis: | Two clones of Escherichia coli O27:K1:H31 and O2:H7, isolated from patients with urinary tract infection or bacteraemia, failed to grow in a synthetic minimal medium (MM) of low osmolality. They were considered to be osmo-remedial because they grew well when sufficient amounts of NaCl, mannitol or sucrose were added to raise the osmolality of the medium to > 300 mOsm/kg. The defect could also be corrected by nicotinamide or its precursors quinolinic and aspartic acids. Each clone had a unique DNA restriction enzyme profile, fimbriae and antibiotic susceptibility patterns. The osmo-remedial variants were unstable and underwent phenotypic modulation to form mixtures with osmo-tolerant forms when grown in MM. They tended to form satellites of small colonies around large colonies of osmo-tolerant cells on MM agar plates. The penicillin method of Davis was used to separate the two forms. Nicotinamide induced the expression of ompF when the osmo-remedial strains were grown under conditions of low osmolality. It is possible that the variants are defective in the synthesis of membrane-derived oligosaccharides or outer-membrane proteins, but this has yet to be determined. |
| Databáze: | OpenAIRE |
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