Interphase Molecular Cytogenetic Detection Rates of Chronic Lymphocytic Leukemia–Specific Aberrations Are Higher in Cultivated Cells Than in Blood or Bone Marrow Smears
| Autor: | Beate Pohle, Rouben Aroutiounian, Cordula Schlie, Anita Glaser, Eyad Alhourani, Tigran Harutyunyan, Thomas Liehr |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty Pathology Histology Chronic lymphocytic leukemia Biology 03 medical and health sciences 0302 clinical medicine Bone Marrow hemic and lymphatic diseases medicine Humans Interphase Cells Cultured In Situ Hybridization Fluorescence Aged Aged 80 and over Chromosome Aberrations medicine.diagnostic_test Cytogenetics Articles Middle Aged medicine.disease Leukemia Lymphocytic Chronic B-Cell Leukemia Lymphatic system medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis Cytogenetic Analysis Female Bone marrow Anatomy 030215 immunology Fluorescence in situ hybridization |
| Popis: | Banding cytogenetics is still the gold standard in many fields of leukemia diagnostics. However, in chronic lymphocytic leukemia (CLL), GTG-banding results are hampered by a low mitotic rate of the corresponding malignant lymphatic cells. Thus, interphase fluorescence in situ hybridization (iFISH) for the detection of specific cytogenetic aberrations is done nowadays as a supplement to or even instead of banding cytogenetics in many diagnostic laboratories. These iFISH studies can be performed on native blood or bone marrow smears or in nuclei after cultivation and stimulation by a suitable mitogen. As there are only few comparative studies with partially conflicting results for the detection rates of aberrations in cultivated and native cells, this question was studied in 38 CLL cases with known aberrations in 11q22.2, 11q22.3, 12, 13q14.3, 14q32.33, 17p13.1, or 18q21.32. The obtained results implicate that iFISH directly applied on smears is in general less efficient for the detection of CLL-specific genetic abnormalities than for cultivated cells. This also shows that applied cell culture conditions are well suited for malignant CLL cells. Thus, to detect malignant aberrant cells in CLL, cell cultivation and cytogenetic workup should be performed and the obtained material should be subjected to banding cytogenetics and iFISH. |
| Databáze: | OpenAIRE |
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