Detection of isoniazid and rifampin resistance of Mycobacterium tuberculosis by a multiplex allele-specific polymerase chain reaction (PCR) assay
| Autor: | Leila Slim-Saidi, Ameni Ghezal, Emna Mehiri, Zeyneb Allegui, Asma Ghariani, Henda Draoui |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty Tuberculosis lcsh:QR1-502 Drug resistance lcsh:Microbiology law.invention Mycobacterium tuberculosis law Multiplex polymerase chain reaction medicine Multidrug-resistant heterocyclic compounds Multiplex Polymerase chain reaction biology business.industry Isoniazid Multiplex PCR biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification medicine.disease Molecular biology Multiple drug resistance Infectious Diseases business Mutations medicine.drug |
| Zdroj: | International Journal of Mycobacteriology, Vol 1, Iss 1, Pp 34-39 (2012) |
| ISSN: | 2212-5531 |
| DOI: | 10.1016/j.ijmyco.2012.01.006 |
| Popis: | Background: The use of molecular techniques is a major improvement for the rapid routine detection and control of multidrug-resistant tuberculosis (MDR-TB). Materials: In this study, the multiplex allele-specific polymerase chain reaction (MAS-PCR) was developed to simultaneously detect the most frequent mutations associated with isoniazid (INH) and rifampin (RIF) resistance in a single assay. Results: The assay was tested with 53 clinical isolates. Among them, 27 were MDR strains, 17 were mono-resistant to INH, one was mono-resistant to RIF, and eight were susceptible. The MAS-PCR assay showed a specificity of 100% in detecting drug resistance. An equivalent sensitivity of 92.6% in detecting MDR and RIF-resistance was found. The sensitivity for the detection of INH-resistance was 88.6%. Conclusions: The MAS-PCR assay was a simple and rapid method for detecting the INH and RIF-resistance in Mycobacterium tuberculosis (MT) clinical strains. It is also easy to perform and to interpret. The assay is inexpensive and a less-demanding technique. |
| Databáze: | OpenAIRE |
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