Minimally Invasive Muscle Embedding Generates Donor-Cell-Derived Muscle Fibers that Express Desmin and Dystrophin
| Autor: | Morium Begam, Mattina M Rosinski, Joseph A. Roche, Sujay S. Galen, Andrea K Eaton, Collin J Elkins, Jaclyn P Johnson |
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| Rok vydání: | 2019 |
| Předmět: |
Myotoxin
Muscle Fibers Skeletal Mice SCID Green fluorescent protein Desmin Extensor digitorum longus muscle Dystrophin 03 medical and health sciences Mice 0302 clinical medicine Tibialis anterior muscle Animals Minimally Invasive Surgical Procedures 030304 developmental biology 0303 health sciences biology Tissue Embedding Myogenesis Chemistry Regeneration (biology) Public Health Environmental and Occupational Health General Medicine Molecular biology Disease Models Animal biology.protein Supplement Article 030217 neurology & neurosurgery |
| Zdroj: | Mil Med |
| ISSN: | 1930-613X |
| Popis: | Introduction The aim of this study was to quantify the extent of donor-cell-derived myogenesis achieved by a novel surgical technique known as Minimally Invasive Muscle Embedding (MIME). Materials and Methods Through MIME, we implanted a single extensor digitorum longus muscle from donor mice (N = 2) that expressed a red fluorescent protein (RFP), into the left tibialis anterior (TA) muscle of immunodeficient host mice (N = 4) that expressed a green fluorescent protein (GFP). Soon after MIME, we injected a myotoxin (barium chloride), into the host TA muscle, to trigger concerted muscle degeneration and regeneration. In lieu of MIME, we performed a SHAM procedure on the right TA muscle of the same set of animals. Results In MIME-treated muscles, 22% ± 7% and 78% ± 7% muscle fibers were RFP+ and GFP+, respectively (mean ± standard deviation); and all RFP+ fibers were positive for desmin and dystrophin. Conclusion. We conclude that MIME helps generate muscle fibers of donor origin, in host muscle. |
| Databáze: | OpenAIRE |
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