Antisense Knockdown of PKC-α Using LNA-Oligos

Autor:	Birgit Giwercman, Henrik Oerum, Majken Westergaard, Jens Bo Hansen, Charlotte Albaek Thrue
Rok vydání:	2003
Předmět:	Lung Neoplasms Protein Kinase C-alpha Transcription Genetic Oligonucleotides Biochemistry Gene Expression Regulation Enzymologic Tumor Cells Cultured Genetics Humans Nucleotide Locked nucleic acid Protein kinase A Protein Kinase C Protein kinase C chemistry.chemical_classification Messenger RNA Gene knockdown Base Sequence Oligonucleotide Cellular Assay General Medicine Oligonucleotides Antisense Thionucleotides Molecular biology chemistry Nucleic acid Molecular Medicine
Zdroj:	Nucleosides, Nucleotides and Nucleic Acids. 22:1607-1609
ISSN:	1532-2335 1525-7770
DOI:	10.1081/ncn-120023045
Popis:	Full-length and 4 nucleotides truncated Locked Nucleic Acid (LNA) modifications of ISIS 3521 were compared for antisense properties in a cellular assay. ISIS 3521 is a 20-mer phosphorothioate designed to hybridise to human protein kinase C-α (PKC-α) mRNA and is currently submitted to clinical trials against cancer. We report that LNA can potentate this antisense oligo and retain the antisense potential with shorter oligos.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d8b68baff9aa2b786401a68f266e663 https://doi.org/10.1081/ncn-120023045 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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