Prevention of Ischemia-Reperfusion Injury with a Synthetic Metalloprotein Superoxide Dismutase Mimic, SC52608
| Autor: | Morton E. Smith, Hong Sp, E. G. Deune, M. R. Ozbek, Roger K. Khouri, Koopman R |
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| Rok vydání: | 1996 |
| Předmět: |
Pathology
medicine.medical_specialty Contraction (grammar) Ischemia Surgical Flaps Superoxide dismutase chemistry.chemical_compound Internal medicine Organometallic Compounds Metalloprotein Animals Medicine Muscle Skeletal HEPES chemistry.chemical_classification Lagomorpha biology business.industry Skeletal muscle Free Radical Scavengers medicine.disease biology.organism_classification Disease Models Animal medicine.anatomical_structure Endocrinology chemistry Reperfusion Injury biology.protein Surgery Rabbits business Reperfusion injury Muscle Contraction |
| Zdroj: | Plastic & Reconstructive Surgery. 98:711-718 |
| ISSN: | 0032-1052 |
| DOI: | 10.1097/00006534-199609001-00018 |
| Popis: | Superoxide dismutase (SOD) scavenges free superoxide radicals generated during reperfusion of ischemic tissue and decreases cellular injury. A synthetic manganese-based metalloprotein superoxide dismutase mimic, SC52608 (Monsanto Co.), was tested in the isolated rabbit rectus femoris muscle flap to determine its effects on ischemia-reperfusion injury. The results of our experiments analyzing 38 isolated rectus femoris muscles in 19 New Zealand White rabbits show that administration of SC52608 at the onset of 4 hours of warm ischemia and before reperfusion significantly increases the survival of the muscle from 20.0 +/- 4.9 percent (control, HEPES) to 81.5 +/- 4.6 percent (SC52608) (p < 0.001). It preserved functional contraction in 8 of 10 muscles; only 1 of 12 control muscles (control, HEPES) had contractions (p = 0.0015). SC52608 decreased the neutrophil density from 4.63 +/- 0.6 x 10(4) cells/mm2 in the control (HEPES) muscle to 2.71 +/- 0.6 x 10(4) cell/mm2 in muscles perfused with SC52608 (p = 0.03). The level of malonyldialdehyde decreased from 6.12 +/- 0.26 nmol/gm (control, HEPES) to 4.64 +/- 0.41 nmol/gm (SC52608) (p = 0.0028). Postoperative weights of the muscles showed no statistical difference (p = 0.14) between the controls (16.0 +/- 0.9 gm) and the SC52608 (18.1 +/- 0.7 gm). Our investigation shows that direct intraarterial infusion of a synthetic superoxide dismutase mimic at the onset of ischemia and prior to reperfusion can reduce reperfusion injury in skeletal muscle. |
| Databáze: | OpenAIRE |
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