Enhancing Flower Color through Simultaneous Expression of the B-peru and mPAP1 Transcription Factors under Control of a Flower-Specific Promoter

Autor: Sangkyu Park, Sun-Hyung Lim, Sun-Hwa Ha, Da-Hye Kim, Jong-Yeol Lee
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Transgene
Nicotiana tabacum
Flowers
01 natural sciences
tobacco
Catalysis
Article
anthocyanin
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
Gene Expression Regulation
Plant
Basic Helix-Loop-Helix Transcription Factors
MYB
Physical and Theoretical Chemistry
Promoter Regions
Genetic
Molecular Biology
Transcription factor
Gene
lcsh:QH301-705.5
Spectroscopy
flower-specific promoter
transcription factor
Regulator gene
Plant Proteins
biology
Basic helix-loop-helix
Pigmentation
flower color
Organic Chemistry
Structural gene
fungi
food and beverages
General Medicine
biology.organism_classification
Plants
Genetically Modified
Molecular biology
Computer Science Applications
030104 developmental biology
Phenotype
lcsh:Biology (General)
lcsh:QD1-999
Organ Specificity
010606 plant biology & botany
Transcription Factors
Zdroj: International Journal of Molecular Sciences, Vol 19, Iss 1, p 309 (2018)
International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 19; Issue 1; Pages: 309
ISSN: 1422-0067
Popis: Flower color is a main target for flower breeding. A transgenic approach for flower color modification requires a transgene and a flower-specific promoter. Here, we expressed the B-peru gene encoding a basic helix loop helix (bHLH) transcription factor (TF) together with the mPAP1 gene encoding an R2R3 MYB TF to enhance flower color in tobacco (Nicotiana tabacum L.), using the tobacco anthocyanidin synthase (ANS) promoter (PANS) to drive flower-specific expression. The transgenic tobacco plants grew normally and produced either dark pink (PANSBP_DP) or dark red (PANSBP_DR) flowers. Quantitative real time polymerase chain reaction (qPCR) revealed that the expression of five structural genes in the flavonoid biosynthetic pathway increased significantly in both PANSBP_DP and PANSBP_DR lines, compared with the non-transformed (NT) control. Interestingly, the expression of two regulatory genes constituting the active MYB-bHLH-WD40 repeat (WDR) (MBW) complex decreased significantly in the PANSBP_DR plants but not in the PANSBP_DP plants. Total flavonol and anthocyanin abundance correlated with flower color, with an increase of 1.6–43.2 fold in the PANSBP_DP plants and 2.0–124.2 fold in the PANSBP_DR plants. Our results indicate that combinatorial expression of B-peru and mPAP1 genes under control of the ANS promoter can be a useful strategy for intensifying flower color without growth retardation.
Databáze: OpenAIRE
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