The major histocompatibility complex class II promoter-binding protein RFX (NF-X) is a methylated DNA-binding protein
| Autor: | Xian-Yang Zhang, Sanja Samac, B M Peterlin, Melanie Ehrlich, Nabila Jabrane-Ferrat, Clement Asiedu |
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| Rok vydání: | 1993 |
| Předmět: |
Transcription
Genetic Genes MHC Class II Molecular Sequence Data Regulatory Factor X Transcription Factors Biology Binding Competitive Methylation DNA-binding protein chemistry.chemical_compound Sequence Homology Nucleic Acid Complementary DNA Tumor Cells Cultured Humans Binding site Promoter Regions Genetic Enhancer Molecular Biology Cell Nucleus Binding Sites Base Sequence Oligonucleotide Binding protein Promoter Cell Biology Molecular biology Recombinant Proteins DNA-Binding Proteins Kinetics Oligodeoxyribonucleotides chemistry Protein Biosynthesis Regulatory Factor X1 DNA HeLa Cells Transcription Factors Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.13.11.6810 |
| Popis: | A mammalian protein called RFX or NF-X binds to the X box (or X1 box) in the promoters of a number of major histocompatibility (MHC) class II genes. In this study, RFX was shown to have the same DNA-binding specificity as methylated DNA-binding protein (MDBP), and its own cDNA was found to contain a binding site for MDBP in the leader region. MDBP is a ubiquitous mammalian protein that binds to certain DNA sequences preferentially when they are CpG methylated and to other related sequences, like the X box, irrespective of DNA methylation. MDBP from HeLa and Raji cells formed DNA-protein complexes with X-box oligonucleotides that coelectrophoresed with those containing standard MDBP sites. Furthermore, MDBP and X-box oligonucleotides cross-competed for the formation of these DNA-protein complexes. DNA-protein complexes obtained with MDBP sites displayed the same partial supershifting with an antiserum directed to the N terminus of RFX seen for complexes containing an X-box oligonucleotide. Also, the in vitro-transcribed-translated product of a recombinant RFX cDNA bound specifically to MDBP ligands and displayed the DNA methylation-dependent binding of MDBP. RFX therefore contains MDBP activity and thereby also EF-C, EP, and MIF activities that are indistinguishable from MDBP and that bind to methylation-independent sites in the transcriptional enhancers of polyomavirus and hepatitis B virus and to an intron of c-myc. |
| Databáze: | OpenAIRE |
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