Genetic Variability and Molecular Evolution of Maize Yellow Mosaic Virus Populations from Different Geographic Origins
| Autor: | San-Ji Gao, Er-Qi He, Sheng-Ren Sun, Hua-Ying Fu, Mei-Ting Huang, Jia-Ju Lu, Jian-Sheng Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine China food.ingredient Genome Viral Plant Science Biology Zea mays 01 natural sciences Maize yellow mosaic virus Evolution Molecular Polerovirus 03 medical and health sciences food Mosaic Viruses Genus Molecular evolution Subject areas Genetic variability Phylogeny Family Luteoviridae Plant Diseases Genetic diversity South America Luteoviridae 030104 developmental biology Evolutionary biology Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant Disease. 105:896-903 |
| ISSN: | 1943-7692 0191-2917 |
| DOI: | 10.1094/pdis-05-20-1013-re |
| Popis: | Maize yellow mosaic virus (MaYMV) hosted in various gramineous plants was assigned to the genus Polerovirus (family Luteoviridae) in 2018. However, little is known about its genetic diversity and population structure. In this study, 509 sugarcane leaf samples with mosaic symptoms were collected in 2017 to 2019 from eight sugarcane-growing provinces in China. Reverse-transcription PCR results revealed that four positive-sense RNA viruses were found to infect sugarcane, and the incidence of MaYMV among samples from Fujian, Sichuan, and Guangxi Provinces was 52.1, 9.8, and 2.5%, respectively. Based on 82 partial MaYMV sequences and 46 whole-genome sequences from different host plants, phylogenetic analysis revealed that MaYMV populations are very closely associated with their source geographical regions (China, Africa, and South America). Pairwise identity analysis showed significant variability in genome sequences among MaYMV isolates with genomic nucleotide identities of 91.1 to 99.9%. In addition to codon mutations, insertions or deletions also contributed to genetic variability in individual coding regions, especially in the readthrough protein (P3-P5 fusion protein). Low gene flow and significant genetic differentiation of MaYMV were observed among the three geographical populations, suggesting that environmental adaptation is an important evolutionary force that shapes the genetic structure of MaYMV. Genes in the MaYMV genome were subject to strong negative or purification selection during evolution, except for the movement protein (MP), which was under positive selection pressure. This finding suggests that the MP may play an important role in MaYMV evolution. Taken together, our findings provide basic information for the development of an integrated disease management strategy against MaYMV. |
| Databáze: | OpenAIRE |
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