Plasma asymmetric dimethylarginine concentrations are not related to differences in maximal oxygen uptake in endurance trained and untrained men

Autor: Julien Boissière, Mehdi Pawlak-Chaouch, Julien Aucouturier, Désiré Munyaneza, Sémah Tagougui, François-Xavier Gamelin, Serge Berthoin, Jean-François Goossens, Amandine Descat, Grégory Cuvelier, Elsa Heyman
Přispěvatelé: Physiotherapy, Human Physiology and Anatomy, Human Physiology and Sports Physiotherapy Research Group, Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 (URePSSS), Université d'Artois (UA)-Université de Lille-Université du Littoral Côte d'Opale (ULCO), Groupe de Recherche sur les formes Injectables et les Technologies Associées - ULR 7365 (GRITA), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille, Université d'Artois (UA)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille, Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), CHU Lille, Univ. Artois, Univ. Littoral Côte d’Opale, Université de Lille, Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369, Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488 [URePSSS], Groupe de Recherche sur les formes Injectables et les Technologies Associées - ULR 7365 [GRITA], Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - EA 7369, Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - EA 7365
Rok vydání: 2018
Předmět:
Adult
Male
medicine.medical_specialty
[SDV]Life Sciences [q-bio]
Endurance Training/methods
Vasodilation
030204 cardiovascular system & hematology
Arginine
Nitric Oxide
medicine.disease_cause
oxygen uptake
cardiovascular biomarker
nitric oxide
Nitric oxide
Incremental exercise
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Exercise/physiology
medicine
Humans
Exercise
Muscles/metabolism
business.industry
Muscles
Physical Endurance/physiology
Skeletal muscle
VO2 max
General Medicine
Oxygen/metabolism
Oxygen
Endurance Training
Endocrinology
medicine.anatomical_structure
chemistry
Nitric Oxide/metabolism
Arginine/analogs & derivatives
Exercise Test
Physical Endurance
Asymmetric dimethylarginine
business
Perfusion
030217 neurology & neurosurgery
Oxidative stress
Zdroj: Experimental physiology
Experimental physiology, 2018, Experimental physiology, ⟨10.1113/EP087398⟩
ISSN: 0958-0670
1469-445X
DOI: 10.1113/ep087398
Popis: NEW FINDINGS: What is the central question of this study? Is there an association of plasma concentration of asymmetric dimethylarginine, which is related to exercise capacity in patients with cardiovascular diseases, with oxygen delivery and subsequently exercise capacity in healthy subjects in the absence of the potentially confounding influence of inflammation and oxidative stress? What is the main finding and its importance? Plasma asymmetric dimethylarginine concentrations are not related to exercise capacity in healthy subjects, while O2 delivery in the working skeletal muscle during the maximal graded-exercise test is not associated with any of the l-arginine analogues. ADMA alone does not play a crucial role in local muscle perfusion and in maintaining exercise capacity. ABSTRACT: Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide (NO) synthesis that could limit oxygen (O2 ) delivery in the working skeletal muscles by altering endothelium-dependent vasodilatation. Exercise capacity is associated with plasma ADMA concentrations in patients with cardiovascular diseases, but this issue has still not been investigated in healthy subjects. We aimed to determine whether plasma ADMA concentrations were negatively associated with exercise capacity in young healthy male subjects. Ten men with maximal oxygen uptake ( V ̇ O 2 max ) > 65 mL kg-1 min-1 were included in the high exercise capacity group (HI-FIT), and 10 men with V ̇ O 2 max < 45 mL kg-1 min-1 were included in the low exercise capacity group (LO-FIT). Plasma ADMA and other l-arginine analogue concentrations were measured before and after a maximal graded-exercise test by liquid chromatography-tandem mass spectrometry. Microvascular O2 delivery during exercise was estimated through the pattern from the sigmoid model of muscle deoxygenation in the vastus lateralis measured by near infrared spectroscopy. V ̇ O 2 max was 60% higher in the HI-FIT group (median: 70.2 mL kg-1 min-1 ; IQR: 68.0-71.9 mL kg-1 min-1 ) than in the LO-FIT group (median: 43.8 mL kg-1 min-1 ; IQR: 34.8-45.3 mL kg-1 min-1 ). Plasma ADMA concentrations did not differ between the LO-FIT and HI-FIT groups before (0.50 ± 0.06 vs. 0.54 ± 0.07 μmol L-1 , respectively) and after the maximal incremental exercise test (0.49 ± 0.08 vs. 0.55 ± 0.03 μmol L-1 ,respectively). There was no significant association of plasma ADMA concentrations with the pattern of local muscle deoxygenation and exercise capacity. Exercise capacity and microvascular O2 delivery are not related to plasma ADMA concentrations in young healthy male subjects. Our findings show that ADMA does not play a crucial role in local muscle perfusion and in maintaining exercise capacity without pathological conditions.
Databáze: OpenAIRE
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