Pyrrolizidine alkaloid clivorine induces apoptosis in human normal liver L-02 cells and reduces the expression of p53 protein
| Autor: | Li-Li Ji, Yu-Chen Sheng, Mian Zhang, Zheng-Tao Wang |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Pyrrolizidine alkaloid
p38 mitogen-activated protein kinases Blotting Western Apoptosis Cell Count Asteraceae Toxicology Cleavage (embryo) Humans Protein kinase A Cells Cultured Pyrrolizidine Alkaloids Polymerase Cell Proliferation Fluorescent Dyes biology Plant Extracts General Medicine Flow Cytometry Molecular biology Cell biology P53 protein Bisbenzimidazole Hepatocytes biology.protein Phosphorylation Poly(ADP-ribose) Polymerases Tumor Suppressor Protein p53 |
| Zdroj: | Toxicology in Vitro. 19:41-46 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2004.06.003 |
| Popis: | Clivorine, a pyrrolizidine alkaloid, is a potent toxic compound extracted from a Chinese medicinal plant Ligularia hodgsonii Hook. We have previously shown that clivorine inhibited human normal liver L-02 cells proliferation and induced p38 mitogen-activated protein kinase phosphorylation. The aim of this study is to further observe the mechanism of clivorine-inhibited L-02 cells growth. In this paper we show here that clivorine can induce apoptosis in L-02 cells, reduce the expression of p53 protein but has no effect on the expression of Bcl-2 protein, and clivorine also induces the cleavage of the poly(ADP-ribose) polymerase in L-02 cells. Our results suggest that the anti-proliferative function of clivorine in L-02 cells may be due to its inducing cell apoptosis, and clivorine-induced cell apoptosis is independent of p53 protein. |
| Databáze: | OpenAIRE |
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