A metagenomic framework for the study of airborne microbial communities
| Autor: | J. Craig Venter, Jeff Hoffman, Lisa Zeigler-Allen, Shannon J. Williamson, Cynthia Andrews-Pfannkoch, Jeffrey B. McQuaid, Johannes B. Goll, Douglas Fadrosh, John I. Glass, Aaron Tenney, Robert Friedman, Shibu Yooseph, Mark Raymond Adams, Mathangi Thiagarajan, Daniel Brami |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Microorganism Biodiversity Air Microbiology lcsh:Medicine Biology 18S ribosomal RNA Microbiology RNA Ribosomal 16S Environmental monitoring Taxonomic rank lcsh:Science Phylogeny Principal Component Analysis Multidisciplinary Bacteria Ecology lcsh:R Bacterial taxonomy Genes rRNA Nucleic acid amplification technique Metagenomics lcsh:Q Nucleic Acid Amplification Techniques Environmental Monitoring Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 12, p e81862 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Understanding the microbial content of the air has important scientific, health, and economic implications. While studies have primarily characterized the taxonomic content of air samples by sequencing the 16S or 18S ribosomal RNA gene, direct analysis of the genomic content of airborne microorganisms has not been possible due to the extremely low density of biological material in airborne environments. We developed sampling and amplification methods to enable adequate DNA recovery to allow metagenomic profiling of air samples collected from indoor and outdoor environments. Air samples were collected from a large urban building, a medical center, a house, and a pier. Analyses of metagenomic data generated from these samples reveal airborne communities with a high degree of diversity and different genera abundance profiles. The identities of many of the taxonomic groups and protein families also allows for the identification of the likely sources of the sampled airborne bacteria. |
| Databáze: | OpenAIRE |
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