The effect of R-(-)-deprenyl administration on antioxidant enzymes in rat testis
| Autor: | Silvia Rybárová, Jozef Mihalik, Peter Solár, Viera Danielisová, Iveta Domoráková, Ingrid Hodorová, Františka Horváthová |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Antioxidant medicine.medical_treatment SOD1 SOD2 Pharmacology Antioxidants Superoxide dismutase 03 medical and health sciences Selegiline Testis medicine Animals Rats Wistar Saline chemistry.chemical_classification biology Chemistry Superoxide Dismutase Catalase Rats 030104 developmental biology Enzyme biology.protein medicine.drug |
| Zdroj: | European journal of pharmacology. 788 |
| ISSN: | 1879-0712 |
| Popis: | The aim of the study was to investigate the effect of R-(-)-deprenyl administration on the activity and localization of superoxide dismutases (SODs) and catalase (CAT) in rat testis. After 30 days of intraperitoneal administration of either saline (control) or R-(-)-deprenyl dissolved in saline at concentrations of 0.0025 mg/kg (low dose of deprenyl, LDD) or 0.25 mg/kg (high dose of deprenyl, HDD), males were killed by thiopental, and their testes were collected. We found that deprenyl administration significantly increased the activity of antioxidant enzymes, and this effect varied by dosage. LDD caused significant elevation of all monitored enzymes, but HDD did not increase the activity of SOD2. Employing immunohistochemistry, we detected enzymes predominantly in Leydig cells (SOD1, SOD2, CAT), in late spermatids and residual bodies (SOD1, SOD2), and in primary spermatocytes (SOD2). Histopathological examination did not reveal testicular damage in experimental groups compared to control. Deprenyl proved to be a potent stimulator of antioxidant enzymes in rat testes; therefore, it could be used in the therapy of male infertility. On the other hand, it is crucial to choose a proper dose, since lower dose was more competent compared to a dosage that was one hundred times higher. |
| Databáze: | OpenAIRE |
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