Effect of hormonal steroids on the uptake of labeled amino acids by HeLa cells
| Autor: | Chiyoko Nakagawa, Tetsuro Mohri, Haruo Kitagawa |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
medicine.medical_specialty
Aminoisobutyric Acids medicine.medical_treatment Steroid HeLa Internal medicine Albumins Drug Discovery medicine Humans Amino Acids Desoxycorticosterone Incubation Lactalbumin chemistry.chemical_classification Carbon Isotopes biology Chemistry Estriol General Chemistry General Medicine biology.organism_classification Amino acid Endocrinology Depression Chemical Glycine Hormone HeLa Cells |
| Zdroj: | Chemicalpharmaceutical bulletin. 19(1) |
| ISSN: | 0009-2363 |
| Popis: | The uptake of glycine-1-14C and AIB-1-14C by HeLa cells into the 60% alcoholsoluble fraction and the effect of steroid (10 μg/ml of medium) on it were studied. After exposure of the cells to cortisol for 48 hr during culture, glycine uptake by the cells in 3, 5, 10 and 60 min of incubation in lactalbumin medium was depressed to 55 to 84% of the control. Larger depressing effect of cortisol on glycine uptake in 10 min of incubation was observed when the cells were incubated in Eagle's medium. Deoxycorticosterone also decreased glycine uptake to 55.6% of the control in 10 min of incubation in Eagle's medium. Cortisol decreased AIB uptake by 33 and 41%, and deoxycorticosterone by 9% (not significant) and 21% in 10 and 30 min of incubation, respectively. 17β-Estradiol and estriol did not significantly affect glycine uptake. |
| Databáze: | OpenAIRE |
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