Retinoic acid receptor-related receptor alpha (RORalpha) is a prognostic marker for hepatocellular carcinoma
| Autor: | Zhigang Zhang, Hu-An Chen, Chun-hui Qiu, Rong-Dang Fu, Min-qiang Lu |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Carcinoma Hepatocellular Retinoic acid Alpha (ethology) Biology chemistry.chemical_compound medicine Biomarkers Tumor Humans Receptor Aged Liver Neoplasms Nuclear Receptor Subfamily 1 Group F Member 1 General Medicine Middle Aged medicine.disease Prognosis Blot Retinoic acid receptor chemistry Hepatocellular carcinoma Cancer research Biomarker (medicine) Immunohistochemistry Female alpha-Fetoproteins Tumor Suppressor Protein p53 |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 35(8) |
| ISSN: | 1423-0380 |
| Popis: | Retinoic acid receptor-related receptor alpha (RORalpha) has been proven to play a tumor suppressive role in certain types of solid tumors. However, the clinical characteristic of RORalpha has not been reported by far. This study investigated the expression of RORalpha in hepatocellular carcinoma (HCC) and evaluated its relationship with clinical parameters and prognosis in HCC patients. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot analyses were performed to detect RORalpha expression levels in 20 paired HCC and corresponding adjacent non-cancerous tissues. Immunohistochemistry was performed on 100 archived paraffin-embedded HCC samples. Statistical analyses evaluated the correlations between RORalpha expression and clinicopathological features. qRT-PCR showed that RORalpha mRNA expression was significantly down-regulated in tumors compared to the adjacent non-cancerous tissues, and Western blots found that RORalpha protein expression was also reduced in tumor tissues. Immunohistochemical assays revealed that decreased RORalpha expression was present in 65 % of HCC patients. Correlation analyses showed that RORalpha expression was significantly correlated with serum alpha fetoprotein (AFP, p = 0.005), pathology grade (p |
| Databáze: | OpenAIRE |
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