A new rat model of prenatal bowel obstruction: development and early assessment
| Autor: | Frédérique Sauvat, Franck Sturtz, Laurent Fourcade, Nadine Cerf-Bensussan, Sabine Sarnacki, Yoanne Mousseau, Naziha Khen-Dunlop |
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| Přispěvatelé: | Biomolécules Thérapies anti-tumorales (EA4021), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503), Service de chirurgie pédiatrique viscérale, orthopédique et plastique [CHU Limoges], CHU Limoges, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Interactions de l'épithelium intestinal avec le système immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Biochimie et Génétique Moléculaire [CHU Limoges], Biomolécules Thérapies anti-tumorales ( EA4021 ), Université de Limoges ( UNILIM ) -Génomique, Environnement, Immunité, Santé, Thérapeutique ( GEIST FR CNRS 3503 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Pathology
MESH : Immunohistochemistry medicine.medical_treatment MESH: Random Allocation [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology [ SDV.MHEP.PED ] Life Sciences [q-bio]/Human health and pathology/Pediatrics MESH : Intestinal Obstruction Gastroenterology Random Allocation 0302 clinical medicine MESH: Pregnancy Pregnancy Reference Values Prenatal Diagnosis Medicine MESH : Female MESH: Animals [ SDV.MHEP.CHI ] Life Sciences [q-bio]/Human health and pathology/Surgery [ SDV.GEN.GH ] Life Sciences [q-bio]/Genetics/Human genetics MESH : Rats Mortality rate Intestinal atresia MESH: Reference Values General Medicine MESH : Survival Rate Immunohistochemistry 3. Good health Bowel obstruction Survival Rate Fetal Diseases MESH: Fetal Diseases [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] [ SDV.NEU.NB ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology 030220 oncology & carcinogenesis Gestation 030211 gastroenterology & hepatology Female [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH : Sensitivity and Specificity medicine.medical_specialty MESH: Rats MESH: Survival Rate MESH: Probability MESH : Probability Rat model [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDV.MHEP.CHI]Life Sciences [q-bio]/Human health and pathology/Surgery MESH : Rats Wistar MESH : Reference Values Sensitivity and Specificity MESH : Random Allocation 03 medical and health sciences Internal medicine [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Animals Rats Wistar MESH: Prenatal Diagnosis MESH : Prenatal Diagnosis Probability Fetus [SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics business.industry Fetal surgery MESH : Fetal Diseases MESH: Immunohistochemistry [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology MESH: Rats Wistar medicine.disease MESH : Disease Models Animal MESH: Sensitivity and Specificity Rats MESH : Pregnancy Disease Models Animal [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Atresia Pediatrics Perinatology and Child Health Surgery MESH : Animals MESH: Disease Models Animal MESH: Intestinal Obstruction business MESH: Female Intestinal Obstruction |
| Zdroj: | Journal of Pediatric Surgery Journal of Pediatric Surgery, American Academy of Pediatrics, 2010, 45 (3), pp.499-506. ⟨10.1016/j.jpedsurg.2009.07.031⟩ Journal of Pediatric Surgery, American Academy of Pediatrics, 2010, 45 (3), pp.499-506. 〈10.1016/j.jpedsurg.2009.07.031〉 |
| ISSN: | 0022-3468 1531-5037 |
| DOI: | 10.1016/j.jpedsurg.2009.07.031⟩ |
| Popis: | International audience; PURPOSE: Although intestinal motility disorders often complicate the postoperative surgical management of newborns with congenital intestinal atresia, their pathogenesis remains unclear. Animal models of prenatal intestinal obstruction have been mainly developed in the lamb and the chicken. Despite new insights brought by these models, they have one or more limitations, such as high fetal mortality rates, high costs, long gestation periods, and an insufficient number of fetuses per litter. Moreover, some species are phylogenetically distant from mammals. METHODS: We developed a reproducible model of prenatal intestinal obstruction in the rat to study the histologic changes induced by the obstruction. We report, the technical devices and the first assessment of this atresia model in a didactic way to allow other researchers to easily reproduce the model. RESULTS: Prenatal intestinal obstructions in this study fulfilled all the macroscopic and histologic criteria usually listed by other models of prenatal intestinal obstruction that have been developed in other species. Furthermore with our model, we obtained a high success rate at a low cost. CONCLUSIONS: We presented in this study a reproducible model of prenatal intestinal obstruction in the rat with the macroscopical and histologic features of prenatal intestinal obstruction. |
| Databáze: | OpenAIRE |
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