IL-22 suppresses the infection of porcine enteric coronaviruses and rotavirus by activating STAT3 signal pathway
Autor: | Hongyan Shi, Jiaoer Zhang, Li Feng, Lan Ying, Pinghuang Liu, Jing Zhao, Mei Xue, Yanlong Ma, Lin Li, Fang Fu |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Rotavirus beta-Defensins Genes Viral Swine medicine.medical_treatment Sequence Homology medicine.disease_cause Interleukin 22 STAT3 0302 clinical medicine Chlorocebus aethiops Cloning Molecular Phylogeny Coronavirus Benzenesulfonates Interleukin-18 Recombinant Proteins Diarrhea Cytokine 030220 oncology & carcinogenesis Cytokines medicine.symptom Coronavirus Infections Signal Transduction STAT3 Transcription Factor Porcine rotavirus Biology Article Rotavirus Infections Microbiology Cell Line 03 medical and health sciences Immune system Immunity Virology medicine Animals Amino Acid Sequence Vero Cells Pharmacology Porcine epidemic diarrhea virus Interleukins Transmissible gastroenteritis virus Epithelial Cells biology.organism_classification Aminosalicylic Acids 030104 developmental biology Gene Expression Regulation Intestinal epithelia Mutation Sequence Alignment |
Zdroj: | Antiviral Research |
ISSN: | 1872-9096 0166-3542 |
Popis: | Interleukin-22 (IL-22), a member of the IL-10 superfamily, plays essential roles in fighting against mucosal microbial infection and maintaining mucosal barrier integrity within the intestine. However, little knowledge exists on the ability of porcine IL-22 (pIL-22) to fight against viral infection in the gut. In this study, we found that recombinant mature pIL-22 (mpIL-22) inhibited the infection of multiple diarrhea viruses, including alpha coronavirus, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine rotavirus (PoRV), in the intestinal porcine epithelial cell line J2 (IPEC-J2) cells. mpIL-22 up-regulated the expression of the antimicrobial peptide beta-defensin (BD-2), cytokine IL-18 and IFN-λ. Furthermore, we found that mpIL-22 induced phosphorylation of STAT3 on Ser727 and Tyr705 in IPEC-J2 cells. Inhibition of STAT3 phosphorylation by S3I-201 abrogated the antiviral ability of mpIL-22 and the mpIL-22-induced expression of BD-2, IL-18, and IFN-λ. Together, mpIL-22 inhibited the infection of PoRV and enteric coronaviruses, and up-regulated the expression of antimicrobial genes in IPEC-J2, which were mediated by the activation of the STAT3 signal pathway. The significant antiviral activity of IL-22 to curtail multiple enteric diarrhea viruses in vitro suggests that pIL-22 could be a novel therapeutic against devastating viral diarrhea in piglets. Highlights • Porcine IL-22 inhibits the most common three porcine diarrhea viruses (rotavirus, PEDV and TGEV) in vitro. • STAT3 signaling by IL-22 accounts for the antiviral activity and the enhanced expression of protective genes of mpIL-22. • IL-22 could provide a novel approach to control devastating viral diarrhea in piglets. |
Databáze: | OpenAIRE |
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