IL-22 suppresses the infection of porcine enteric coronaviruses and rotavirus by activating STAT3 signal pathway

Autor: Hongyan Shi, Jiaoer Zhang, Li Feng, Lan Ying, Pinghuang Liu, Jing Zhao, Mei Xue, Yanlong Ma, Lin Li, Fang Fu
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Rotavirus
beta-Defensins
Genes
Viral
Swine
medicine.medical_treatment
Sequence Homology
medicine.disease_cause
Interleukin 22
STAT3
0302 clinical medicine
Chlorocebus aethiops
Cloning
Molecular
Phylogeny
Coronavirus
Benzenesulfonates
Interleukin-18
Recombinant Proteins
Diarrhea
Cytokine
030220 oncology & carcinogenesis
Cytokines
medicine.symptom
Coronavirus Infections
Signal Transduction
STAT3 Transcription Factor
Porcine rotavirus
Biology
Article
Rotavirus Infections
Microbiology
Cell Line
03 medical and health sciences
Immune system
Immunity
Virology
medicine
Animals
Amino Acid Sequence
Vero Cells
Pharmacology
Porcine epidemic diarrhea virus
Interleukins
Transmissible gastroenteritis virus
Epithelial Cells
biology.organism_classification
Aminosalicylic Acids
030104 developmental biology
Gene Expression Regulation
Intestinal epithelia
Mutation
Sequence Alignment
Zdroj: Antiviral Research
ISSN: 1872-9096
0166-3542
Popis: Interleukin-22 (IL-22), a member of the IL-10 superfamily, plays essential roles in fighting against mucosal microbial infection and maintaining mucosal barrier integrity within the intestine. However, little knowledge exists on the ability of porcine IL-22 (pIL-22) to fight against viral infection in the gut. In this study, we found that recombinant mature pIL-22 (mpIL-22) inhibited the infection of multiple diarrhea viruses, including alpha coronavirus, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine rotavirus (PoRV), in the intestinal porcine epithelial cell line J2 (IPEC-J2) cells. mpIL-22 up-regulated the expression of the antimicrobial peptide beta-defensin (BD-2), cytokine IL-18 and IFN-λ. Furthermore, we found that mpIL-22 induced phosphorylation of STAT3 on Ser727 and Tyr705 in IPEC-J2 cells. Inhibition of STAT3 phosphorylation by S3I-201 abrogated the antiviral ability of mpIL-22 and the mpIL-22-induced expression of BD-2, IL-18, and IFN-λ. Together, mpIL-22 inhibited the infection of PoRV and enteric coronaviruses, and up-regulated the expression of antimicrobial genes in IPEC-J2, which were mediated by the activation of the STAT3 signal pathway. The significant antiviral activity of IL-22 to curtail multiple enteric diarrhea viruses in vitro suggests that pIL-22 could be a novel therapeutic against devastating viral diarrhea in piglets.
Highlights • Porcine IL-22 inhibits the most common three porcine diarrhea viruses (rotavirus, PEDV and TGEV) in vitro. • STAT3 signaling by IL-22 accounts for the antiviral activity and the enhanced expression of protective genes of mpIL-22. • IL-22 could provide a novel approach to control devastating viral diarrhea in piglets.
Databáze: OpenAIRE
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