Recovery potential in glucose deprived astrocytes
| Autor: | Josianne Lacremans-Pirsoul, Georges Baudoux, Jeannine Mertens-Strijthagen |
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| Rok vydání: | 1996 |
| Předmět: |
Tris
Male medicine.medical_specialty chemistry.chemical_element Oxidative phosphorylation Biology Deoxyglucose Creatine Oxygen Incubation period Rats Sprague-Dawley chemistry.chemical_compound Adenosine Triphosphate Oxygen Consumption Internal medicine medicine Animals Cells Cultured HEPES Cerebral Cortex Cell Death General Neuroscience Substrate (chemistry) General Medicine Carbon Dioxide Phosphate Rats Endocrinology Glucose chemistry Astrocytes |
| Zdroj: | Neuroscience research. 26(2) |
| ISSN: | 0168-0102 |
| Popis: | d -glucose deprivation for a 45 min period reduces the ATP and creatine phosphate concentrations of astrocytes. Recovery experiments were initiated by reincubating the cells with d -glucose and glucose replacement metabolites. No recovery of ATP concentration could be obtained even after 1 h of reincubation with the replacement metabolites. After a 45 min incubation period without d -glucose, 14 CO 2 production fell to 36% and 21% of controls when the cells were reincubated respectively with d -[U- 14 C]-glucose and l -[2- 14 C]-pyruvate as substrate marker. When reincubated for 1 h in the presence of l -malate (1 mM) + l -pyruvate (10 mM) with l -[2- 14 C]-pyruvate as marker, a total recovery of 14 CO 2 production was ascertained. Reincubation of the glucose deprived cells in the presence of d -glucose (10 mM) did not increase the 14 CO 2 production indicating that the cells were unable to use d -glucose for oxidative purposes. As pyruvate concentration was dramatically decreased in glucose deprived cells, astrocytes were treated with α-ketovalerate (25 mM) which led to an 8-fold increase in pyruvate concentration. In these conditions 14 CO 2 production did not increase when the cells were incubated in the presence of l -malate (1 mM). O 2 consumption of State 4 in astrocytes, submitted to glucose deprivation, decreased. These cells treated with FCCP could not be uncoupled and when reincubated in the presence of replacement metabolites only a 20% increase of oxygen consumption took place. |
| Databáze: | OpenAIRE |
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