Desmin filaments studied by quasi-elastic light scattering
| Autor: | Melanie Hohenadl, Hulda Kirpal, Klaus Kroy, Rudolf Merkel, Tobias Storz |
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| Předmět: |
Light
Polymers Intermediate Filaments Magnesium Chloride Biophysics macromolecular substances Sodium Chloride Molecular physics Light scattering Desmin Protein filament Animals Scattering Radiation Intermediate filament Persistence length Viscosity Chemistry Scattering Osmolar Concentration Type III Intermediate Filament Temperature Elasticity Kinetics Microscopy Electron Crystallography Models Chemical Polymerization Gizzard Avian Chickens Mathematics Research Article |
| Zdroj: | ResearcherID |
| Popis: | We studied polymers of desmin, a muscle-specific type III intermediate filament protein, using quasi-elastic light scattering. Desmin was purified from chicken gizzard. Polymerization was induced either by 2 mM MgCl(2) or 150 mM NaCl. The polymer solutions were in the semidilute regime. We concluded that the persistence length of the filaments is between 0.1 and 1 microm. In all cases, we found a hydrodynamic diameter of desmin filaments of 16-18 nm. The filament dynamics exhibits a characteristic frequency in the sense that correlation functions measured on one sample but at different scattering vectors collapse onto a single master curve when time is normalized by the experimentally determined initial decay rate. |
| Databáze: | OpenAIRE |
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