Features of Postoperative Immune Suppression Are Reversible With Interferon Gamma and Independent of Interleukin-6 Pathways
| Autor: | Michael J. O’Dwyer, Charles J. Hinds, Helen C. Owen, Hew D.T. Torrance, E. Rebecca Longbottom, Paraskevi C. Fragkou, Rupert M Pearse |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Peripheral blood mononuclear cell 03 medical and health sciences Interferon-gamma 0302 clinical medicine Immune system Postoperative Complications Immune Tolerance Medicine Humans Interferon gamma Interleukin 6 Digestive System Surgical Procedures Aged Aged 80 and over biology business.industry Interleukin-6 Monocyte Age Factors Interleukin Perioperative Middle Aged 030104 developmental biology medicine.anatomical_structure Elective Surgical Procedures 030220 oncology & carcinogenesis Case-Control Studies Immunology biology.protein Surgery Female Antibody business medicine.drug |
| Zdroj: | Annals of surgery. 264(2) |
| ISSN: | 1528-1140 0003-4932 |
| Popis: | OBJECTIVE\ud The aim of this study was to evaluate the role of interleukin (IL)-6 pathways in postoperative immune suppression and to assess the reversibility of this phenomenon.\ud \ud BACKGROUND\ud The postoperative period is characterized by increased IL-6 production and features of immune suppression. In vitro, IL-6 mediates anti-inflammatory effects through inhibition of interferon gamma (IFN-γ) pathways. The significance of the immunomodulatory effects of IL-6 in the clinical setting of postoperative immune suppression remains unclear.\ud \ud METHODS\ud Patients over 45 years old undergoing elective surgery, involving the gastrointestinal tract, were recruited. IL-6 levels were assayed using an enzyme linked immunosorbent assay preoperatively, and at 24 and 48 hours. Peripheral blood mononuclear cells from healthy volunteers were cultured in perioperative serum and CD14Human Leukocyte Antigen-DR (HLA-DR) [monocyte HLA-DR (mHLA-DR)] geometric mean florescent intensity was measured in the presence and absence of IL-6 neutralizing antibody and recombinant IFN-γ.\ud \ud RESULTS\ud Of the 108 patients, 41 developed a postoperative infection. The IL-6 levels increased 19-fold from the preoperative sample to 24 hours postoperatively (P |
| Databáze: | OpenAIRE |
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