Mutational and Bioinformatic Analysis of Haloarchaeal Lipobox-Containing Proteins
| Autor: | Zhongqiang Chen, Saheed Imam, Kieran Dilks, Mechthild Pohlschroder, Friedhelm Pfeiffer, Stefanie Storf |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Signal peptide
Proteomics Article Subject Physiology In silico Archaeal Proteins Lipoproteins Molecular Sequence Data Sequence alignment Protein Sorting Signals Signal peptide processing Microbiology Genes Archaeal Amino Acid Sequence Peptide sequence Haloferax volcanii Ecology Evolution Behavior and Systematics Genetics biology Membrane transport protein Cell Membrane Computational Biology Membrane Transport Proteins biology.organism_classification QR1-502 Transport protein Protein Transport Mutation biology.protein Sequence Alignment Research Article |
| Zdroj: | Archaea Archaea, Vol 2010 (2010) |
| ISSN: | 1472-3654 1472-3646 |
| Popis: | A conserved lipid-modified cysteine found in a protein motif commonly referred to as a lipobox mediates the membrane anchoring of a subset of proteins transported across the bacterial cytoplasmic membrane via the Sec pathway. Sequenced haloarchaeal genomes encode many putative lipoproteins and recent studies have confirmed the importance of the conserved lipobox cysteine for signal peptide processing of three lipobox-containing proteins in the model archaeonHaloferax volcanii. We have extended thesein vivoanalyses to additionalHfx. volcaniisubstrates, supporting our previousin silicopredictions and confirming the diversity of predictedHfx. volcaniilipoproteins. Moreover, using extensive comparative secretome analyses, we identified genes encodining putative lipoproteins across a wide range of archaeal species. While ourin silicoanalyses, supported byin vivodata, indicate that most haloarchaeal lipoproteins are Tat substrates, these analyses also predict that many crenarchaeal species lack lipoproteins altogether and that other archaea, such as nonhalophilic euryarchaeal species, transport lipoproteins via the Sec pathway. To facilitate the identification of genes that encode potential haloarchaeal Tat-lipoproteins, we have developed TatLipo, a bioinformatic tool designed to detect lipoboxes in haloarchaeal Tat signal peptides. Our results provide a strong foundation for future studies aimed at identifying components of the archaeal lipoprotein biogenesis pathway. |
| Databáze: | OpenAIRE |
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